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Development of Immunological Procedure for Prevention of Dental Caries Using Fusion Protein of Colonization Factors

Research Project

Project/Area Number 07557134
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 矯正・小児・社会系歯学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KOGA Toshihiko  KYUSHU UNIVERSITY,Faculty of Dentistry, Professor, 歯学部, 教授 (10037541)

Co-Investigator(Kenkyū-buntansha) YU Ho  KYUSHU UNIVERSITY,Faculty of Dentistry, Research Associate, 歯学部, 助手 (30260714)
NAKANO Yoshio  KYUSHU UNIVERSITY,Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (80253459)
OHO Takahiko  KYUSHU UNIVERSITY,Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (50160940)
中井 素行  ライオン(株)オーラルケア研究所, 研究員
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥9,500,000 (Direct Cost: ¥9,500,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsDental Caries / Streptococcus mutans / Colonization Factor / Fusion Protein / Passive Immunization / Protein Antigen / Glucosyltransferase / Prevention of Dental Caries
Research Abstract

Cell-surface protein antigen (PAc) and glucosyltransferases (GTFs) produced by Streptococcus mutans are considered to be major colonization factors of the organism, and the inhibition of these two factors is predicted to provide protection against dental caries. In this study, we have constructed a fusion protein PAcA : GB of the salivabinding alanine-rich region (PAcA) of PAc and the glucan-binding (GB) domain of GTF-I,an enzyme catalyzing the synthesis of water-insoluble glucan from sucrose, and a fusion protein PAcA : SB of PAcA and the sucrose-binding (SB) domain of GTF-I.The recombinant fusion proteins were purified from cell extracts of Escherichia coli harboring the fusion genes, and rabbit antibodies against these fusion proteins were prepared. The water-insoluble glucan synthesis by cell-associated and cell-free GTF preparations from S.mutans and the total glucan synthesis by GTF-I was markedly inhibited by anti-PAcA : GB IgG antibodies, but not by anti-PAcA : SB IgG antibodies. Significant inhibition of the sucrose-independent and sucrose-dependent adhesion of S.mutans to saliva-coated hydroxyapatite beads was observed when anti-PAcA : GB antibodies were added to the reaction mixture. Anti-PAcA : SB antibodies inhibited the adhesion of S.mutans to the beads in the absence of sucrose, but did not in the presence of sucrose. Immunization with the fusion protein PAcA : GB may be useful for controlling the colonization of teeth by the organism.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Murakami, Y.他3名: "Identification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans strain GS-5." Infect.Immun.65. 794-797 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsukioka, Y.他4名: "Biological function of the dTDP-rhamnose synthesis pathway in Streptococcus mutans" J.Bacteriol.179. 1126-1134 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murakami, Y.他5名: "Role of the charged tail on localization of a surface protein antigen of Streptococcus mutans" Infect.Immun.65. 1531-1535 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsukioka, Y.他4名: "Identification of a fourth gene concerned with dTDP-rhamnose synthesis in Streptococcus mutans." J.Bacteriol.179. 4411-4414 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] YU, H.他4名: "Effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans" Infect.Immun.65. 2292-2298 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oho, T.他3名: "Binding of salivary glycoprotein-secretory immunoglobulin A complex to the surface protein antigen of Streptococcus mutans." Infect.Immun.66. 115-121 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 古賀 敏比古 他36名: "う蝕細菌の分子生物学" 岩波書店, 360 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murakami, Y.et al.: "Indentification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans strain GS-5" Infect.Immun. 65. 794-797 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsukioka, Y.et al.: "Biological function of the dTDP-rhamnose synthesis pathway in Streptococcus mutans" J.Bacteriol. 179. 1126-1134 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murakami, Y.et al.: "Role of the charged tail on localization of a surface protein antigen of Streptococcus mutans" Infect.Immun. 65. 1531-1535 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yu, H.et al.: "Effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans" Infect.Immun. 65. 2292-2298 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsukioka, Y.et al.: "Indentification of a fourth gene concerned with dTDP-rhamnose synthesis in Streptococcus mutans.J.Bacteriol.179 : 4411-4414,1997." J.Bacteriol. 179. 4411-4414 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oho.T.et al.: "Binding of salivary glycoproteinsecretory immunoglobulin A complex to the surface protein antigen of Streptococcus mutans" Infect.Immun. 66. 115-121 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murakami,Y.他3名: "Identification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans strain GS-5." Infect.Immun.65. 794-797 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsukioka,Y.他4名: "Biological function of the dTDP-rhamnose synthesis pathway in Streptococcus mutans" J.Bacteriol.179. 1126-1134 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murakami,Y.他5名: "Role of the charged tail on localization of a surface protein antigen of Streptococcus mutans" Infect.Immun.65. 1531-1535 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsukioka,Y.他4名: "Identification of a fourth gene concerned with dTDP-rhamnose synthesis in Streptococcus mutans." J.Bacteriol.179. 4411-4414 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yu,H.他4名: "Effects of antibodies against cell surface protein antigen PAc-glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans" Infect.Immun.65. 2292-2298 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Oho,T.他3名: "Binding of salivary glycoprotein-secretory immunoglobulin A complex to the surface protein antigen of Streptococcus mutans." Infect.Immun.66. 115-121 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 古賀敏比古 他36名: "う蝕細菌の分子生物学" 岩波書店, 360 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Senpuku,H.: "Identification of a repeated epitope recognized by human serum antibodies in a surface protein antigen of Streptococcus mutans" Oral Microbiol.Immunol.11. 121-128 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamashita,Y.: "Molecular and genetic analysis of multiple changes in the levels of production of virulence factors in subcultured variant of Streptococcus mutans" FEBS Microbiol.Lett.144. 81-87 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Senpuku,H.: "Identification of human antigenic epitopes in an alanine-rich repeating region using sera from hu-PBL-SCID mice immunized with a surface protein antigen of Streptococcus mutans" Oral Microbiiol.Lett.5. 343-349 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Murakami,Y.: "Identification of a frameshift mutation resulting in premature termination and loss of cell wall anchoring of the PAc antigen of Streptococcus mutans strain GS-5" Infect.Immun.65. 794-797 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 古賀 敏比古: "齲蝕免疫" 歯界展望. 88. 1035-1046 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hajishengallis,G.: "Mucosal immunization with a bacterial protein antigen genetically coupled to cholera toxin A2/B subunits." Journal of Immunology. 154. 4322-4332 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakano,Y.: "Construction of a series of pACYC-derivative vectors." Gene. 162. 157-158 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koga,T.: "Surface proteins of Streptococcus mutans." Dev.Biol.Stand.Basel,Karger. 85. 363-369 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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