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Establishment of gene therapy vector by using DNA replication origin and virus vector

Research Project

Project/Area Number 07557147
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biological pharmacy
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ARIGA Hiroyoshi  Hokkaido Univ., Fac.of Pharm., Prof., 薬学部, 教授 (20143505)

Co-Investigator(Kenkyū-buntansha) ARIGA Sanae  Hokkaido Univ., Coll.of Med.Tech., Prot., 医療技術短期大学部, 教授 (90184283)
UEDA Masatugu  YS New Technology Ins., Direc., 所長
上田 正次  雪印乳業(株), 生物科学研究所, 主任研究員
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 1997: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1996: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsMYC / adenovirus / vector / heat shock protein / transcription / DNA replication / c-myc / DNA複製開始 / 結合タンパク質 / 癌遺伝子 / DNA結合 / 転写因子 / 発現ベクター / 細胞周期
Research Abstract

Expression vector composed of adenovirus, beta-galactosidase, and DNA replication origin (ori) was constricted.Oris used here were from genes of c-myc, human hsp70 and immunoglobulin heavy chain in addition to SV40 origin as a positive control. These origins were inserted to adenovirus vector containing Cre-lox system, and the recombinant adenovirus were recovered from human 2983 cells transfected with these vectors. Adenovirus containing SV40 origin were first infected to monkey CosI cells, and the circular adenovirus expressing beta-galactosidase was recovered. Adenovirus containing hsp70 gene origin were then infected to human HeLa cells, and the circular form of adenovirus DNA was also recovered. Copy number of hsp70 ori derived plasmid DNA in the cells, however, was lower than that of SV40 origin in 10^<-3> order. Results suggest that this system should be useful for an expression vector for gene therapy.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Pognonec, P: "Cross-family interaction between the bHLHZip USF and bZip Fral proteins o" Oncogene. 14. 2091-2098 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M.: "Effect of transcriptional regulatory sequences on autonomous replication of" Biol.Pharm.Bull.20. 613-620 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Beppu, Y.: "Human mucus protease inhibitor in airway fluids is a potential defensiv" J.Biochem.121. 309-316 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagakubo, D.: "DJ-1,a novel oncogene product which transforms mouse NIH3T3 cells in" Biochem.Biophy.Res.Comm.231. 509-513 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taira, T.: "A novel G1/S-specific enhancer identified in the human heat shock protein 70" Nucleic Acids Res.25. 1975-1983 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iida, M.: "Induction of apoptosis by MSSP,C-MYC binding proteins." Biol.Pharm.Bull.20. 10-14 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 有賀 寛芳: "癌遺伝子研究の展望2" 東京化学同人, 250 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 有賀 寛芳: "最新の分離・精製・検出法" エヌ・テイー・エス, 850 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Pognonec, P.: "Cross-family interaction between the bHLHZip USF and bZip Fral protei" Oncogene. 14. 2091-29098 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokoyama, M.: "Effect of transcriptional regulatory sequences on autonomous replication" Biol.Pharm.Bull.20. 613-620 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Beppu, Y.: "Human mucus protease inhibitor in airway fluids is a potential defensive" J.Biochem.121. 309-316 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagakubo, D.: "DJ-1, a novel oncogene product which transforms mouse NIH3T3 cells in" Biochem.Biophy.Res.Comm.231. 509-513 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taira, T.: "A novel G1/S-specific enhancer identified in the human heat shock protein" Nucleic Acids Res.25. 1975-1983 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] lida, M.: "Induction of apoptosis by MSSP,C-MYC binding proteins." Biol.pharm.Bull.20. 10-14 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariga, H.: Perspective of oncogene II.Tokoy Kagaku Dojin, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ariga, H.: Method of purification and analyis.NTN, (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Pognonec,P: "Cross-family interaction between the bHLHZip USF and bZip Fral proteins results in down regulation of apt activity" Oncogene. 14. 2091-2098 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yokoyama,M.: "Effect of transcriptional regulatory sequences on autonomous replication of Plasmidsin Transient Mammalion Systems" Biol.Pharm. Bull.20. 613-620 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Beppu,Y.: "Human mucus protease inhibitor in airway fluids is a potential defensiv" J.Biochem.121. 309-316 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagakubo,D.: "DJ-1,a novel oncogene product which transforms mouse NIH3T3 cells in" Biochem.Biophy.Res.Comm.231. 509-513 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Taira,T.: "A novel G1/S-specific enhancer identified in the human heat shock protein 70 Gene" Nucleic Acids Res.25. 1975-1983 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Iida,M.: "Induction of apoptosis by MSSP,C-MYC binding proteins." Biol.Pharm.Bull.20. 10-14 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 有賀寛芳: "癌遺伝子研究の展望2" 東京化学同人, 250 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 有賀寛芳: "最新の分離・精製・検出法" エヌ・テイー・エス, 850 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Manabe, A: "Transcriptional repression activity of N-myc protein requires phosphorylation by MAP kinase." Biochem. Biophys. Res. Comm.219. 813-823 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Haigermoser, C.: "Cloning and characterization of the genomic DNA of the human MSSP gene." Nucleic Acids Res.24. 3856-3857 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iida, M.: "Induction of apoptosis by MSSP, C-MYC binding proteins." Biol. Pharm. Bull. 20. 10-14 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taira, T.: "A novel Gl/S-speciffic enhancer identified in the human heat shock protein 70 gene" J. Biol. Chem. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuchiya, T.: "ku antigen binds to Alu family DNA and represses transcription." J. Biol. Chem.(in press.). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda, J.: "AMY-1, a novel C-MYC binding proterin that stimulates transcription and transforming activities of C-MYC" Nature. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Izumi, S.: "Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation." Biochim.Biophys.Acta. 1267. 135-138 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Iwata, K.: "Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane confactor protein CD46." J. Biol. Chem.270. 15148-15152 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Taira, T.: "Autonomously replicating segment identified in the murine p53 gene contains p53 recognition sequence and bending region." Int. J. Onc.7. 115-122 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi, C.: "Involvement of PIM-1 in DNA fragmentation in mous NS-1-derived cells." Biochem Biophys. Res. Commun.215. 538-546 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Manabe, A.: "Transcriptional repression activity of N-myc protein requires phosphorylation by MAP kinase." Biochim Biophys. Res. Commun.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 有賀寛芳: "神経細胞の増殖・分化に関与するmyc遺伝子とアポトーシス" 学術月報. 48. 1036-1041 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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