| Project/Area Number |
07557178
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Laboratory medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
ARAI Toshiyuki Kyoto University, Medicine, Associated Professor, 医学研究科, 助教授 (80175950)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Koji Shimadzu Corporation, MRI,Chief engineer, 医用応用技術部, 技師
ISHIKAWA Masatsune Kyoto University, Medicine, Associated Professor, 医学研究科, 助教授 (20115786)
MORI Kenjiro Kyoto University, Medicine, Professor, 医学研究科, 教授 (20025620)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥16,100,000 (Direct Cost: ¥16,100,000)
Fiscal Year 1996: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1995: ¥11,000,000 (Direct Cost: ¥11,000,000)
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| Keywords | cerebral blood flow / cerebral metabolic rate for oxygen / magnetic resonance imaging / water-17 / oxygen-17 / 中大脳動脈閉塞 / 脳梗塞 / 組織標本 / 神経細胞 / 神経膠細胞 |
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| Research Abstract |
A method for simultaneous measurements of cerebral blood flow (CBF) and cerebral metabolic rate for oxygen (CMRo_2) by magnetic resonance imaging (MRI) was developed.
First, CBF was measured in cats by MRI,using ^<17>O-labeled water (water-17) as a tracer. Cat brain images were obtained using a fast spin-echo sequence. Intravenous infusion of water-17 and collection of a series of arterial blood samples were simultaneously performed during the MRI scan. From changes in brain signal intensity induced by water-17 infusion, water-17 concentration of brain were calculared. Water-17 concentration in arterial blood was measured directly by ^<17>O NMR spectroscopy. From these data, CBF was successfully determined using an in vivo autoradiographic strategy.
Next, CBF and CMRo_2 were measured simultaneously in cats by MRI,using ^<17>O-labeled oxygen gas (oxygen-17 gas) as a tracer. Cat brain images were obtained using a fast spin-echo sequence. During inhalation of oxygen-17 gas, a series of MRI scans and arterial blood samplings were performed. From changes in brain signal intensity induced by oxygen-17 inhalation, metabolic water-17 concentration of brain were calculated. Metabolic water-17 concentration in arterial blood was measured directly by ^<17>O NMR spectroscopy. Applying convolution integral and least square method, CBF and CMRo_2 were simultaneously calculated from these data. However, the obtained values were not sufficient as reasonable values. To resolve this problem, changes in signal intensity, induced by oxygen-17 gas inhalation, should be detected much more sensitively by MRI.
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