| Project/Area Number |
07557203
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 試験 |
|---|
| Research Field |
寄生虫学(含医用動物学)
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
MINAGAWA Tomonori Hokkaido University School of Medicine Professor, 医学部, 教授 (40001903)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ISHIWATA Kenji Hokkaido University School of Medicine Lecturer, 医学部, 助手 (00241307)
SATOH Yu-ichiro Hokkaido University School of Medicine Lecturer, 医学部, 助手 (00291229)
SATOH Naoki Hokkaido University School of Medicine Assistant Prof., 医学部・附属病院, 助教授 (70205946)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Echinococcosis / Echinococcus multilocularis / Host-parasite relationship / Cytokines / IFN-gamma / Immunotherapy / BRM-therapy / 宿主寄生体相互関係 / エキノコックス / 多包虫症 / 肝臓 / IL-6 / TNF-α |
|---|
| Research Abstract |
Patient with the early stage of alveolar echinococcosis are treated by partial surgical resection of infected liver. They are cured completely by ones with inoperable lesions and metastatic lesions other than liver are treated only by hopeless drugs. Therefore, we want to treat them by specific immunotherapy or nonspecific biological response modifiers. However, the immunological status of the patiens with echinococcosis has not been investigated so far. The purpose of our project is to make clear the host-parasite relationship of patients with echinococcosis.
1.High level of gamma-IFN was detected in the extract of unaffected parts of livers obtained from 10 patients with echinococcosis.
2.IL-6 and TNF-alpha were detected only in the infected lesions of livers.
3.mRNA of cytokines were investigated by RT-PCR.mRNA of gamma-IFN was detected in the extracts of unaffected parts of livers obtained from normal or other disorders were shown to contain undetectable level of gamma-IFN.
The most important problem of this project is that the number of patients to be investigated was very few and the results between titers of gamma-IFN assayd by ELISA and the detection of mRNA of gamma-IFN were often different. We must concentrate the improvement of the assay system of cytokines and accumulate the number of patients with echinococcosis.
|
