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Development of methods for specific detection of oligosaccharide chains of rheumatoid factor in sera from patients with early-phase rheumatoid arthritis.

Research Project

Project/Area Number 07557226
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 内科学一般
Research InstitutionTokai University, School of Engineering

Principal Investigator

MIZUOCHI Tsuguo  Tokai University, School of Engineering, Professor, 工学部, 教授 (90133149)

Co-Investigator(Kenkyū-buntansha) NAKATA Munehiro  Tokai University, School of Engineering, Associate Professor, 工学部, 助教授 (00266371)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsrheumatoid arthritis / rheumatoid factor / immunoglobulin G / early-phase rheumatoid arthritis / serum test / diagnosis of rheumatoid arthritis / abnormality in oligosaccharide structures / レクチン
Research Abstract

In the present study, IgG was highly purified from pooled sera of healthy volunteers. Galactose-free IgG was then prepared from the human IgG by enzymatic oligosaccharide-remodelling. Analyzes of the protein moiety and oligosaccharide structures of both intact IgG and the oligosaccharide-remodelled IgG indicated that the oligosaccharide-remodelling completely removed galactose residues of IgG without damaging the protein moiety. Using this galactose-free IgG,novel serum assay system for detection of rheumatoid factor was designed as follows. The galactose-free IgG was immobilized on a 96-well microtiter plate, to which serum sample was added. If rheumatoid factor exists in the serum sample, it binds to galactose-free IgG on the plate. Them galactose residues of rheumatoid factors bound to the galactose-free IgG were detected with HRP conjugated lectin. Conditions for immobilizing the galactose-free IgG on the plate, a kind and concentration of the lectin, detergent, and blocking reagent, amount of serum sample analyzed, reaction temperature, and reaction time were investigated in detail. Finally, an optimal assay condition was established for specific detection of oligosaccharides of rheumatoid factor in sera from patients with rheumatoid arthritis. When serum samples from healthy individuals, patients with rheumatoid arthritis, rheumatic diseases, and the other diseases were analyzed using this novel serum test, it was indicated that this novel serum test is superior to conventional serum tests (RA test and RAHA test) for detection of rheumatoid factor in terms of specific detection of patients with rheumatoid arthritis including seronegative patients and early-phase patients and understanding of the disease activity in patients. Therefore, this novel serum test could be very useful for accurate diagnosis and treatment of this disease.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Sugimoto, M.: "Oligomannose-coated liposomes as an adjuvant fot the induction of cell-modiated immunity." FEBS Lett.363. 53-56 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "Oligosaccharide-coated liposomes : a strategy of AIDS vaccine with emphasis on callular immunity." Glycoconjugate J.12. 584-584 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中田 宗宏: "免疫グロブリンの糖鎖構造" 臨床検査. 39. 636-642 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 水落 次男: "HIV表面糖鎖結合新型エイズ治療薬の開発" 日本臨床. 53. 2340-2349 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中田 宗宏: "リウマトイド因子と糖鎖構造" 診断と治療. 83. 1173-1177 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsushita, M.: "A novel human serum lectin with collagen-and fibrinogen-like domains functions as an opsonin." J.Boil.Chem.271. 2448-2454 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "Neoglycolipid as a safe adjuvant for induction of cytotoxic T lymphocyte." AIDS Research Newsletter. 10. 172- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 水落 次男: "慢性関節リウマチにおけるガラクトース欠損IgGの病因的意義" 最新医学. 51. 2354-2359 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Moriwaki, T.: "Alteration of N-linked oligosaccharide structures of human chorionic gonadotiopin β-subunit by disruption of disulfide bonds." Glycoconjugate J.14. 225-229 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "Role of galactosylation in the renal pathogenicity of musine IgG3 monoclonal antibodies." Glycoconjugate J.14 supliment. S138- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中田 宗宏: "糖鎖異常IgGとマンノース結合蛋白質による補体系の活性化" 臨床免疫. 29. 162-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中田 宗宏: "慢性関節リウマチにおけるIgG糖鎖異常の意義" 医学のあゆみ. 182. 568-572 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shikata, K.: "Structual changes in the oligosacchariole moiety of human IgG with aging." Glycoconjugate J.(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中田 宗宏: "リウマチ'95「RA診断におけるリウマチ因子糖鎖測定の意義」" メディカルレビュー社, 96-102 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 志方 幸道: "リウマチ病セミナIV「慢性関節リウマチにおける糖鎖異常」" 永井書店, 184-193 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 水落 次男: "グライコバイオロジー実験プロトコール「糖タンパク質糖鎖を導入した人工糖脂質とのDirect Binding Assoy」" 秀潤社, 42-47 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 水落 次男: "糖鎖生物学「HIVと糖鎖」" 共立出版(印刷中), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugimoto, M.: "Oligomannose-coated liposomes as an adjuvant for the induction of cell-mediated immunity." FEBS Lett.363. 53-56 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "Oligosaccharide-coated liposomes : a strategy of AIDS vaccine with emphasis on cellular immunity." Glycoconjugate J.12. 584 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakata, M.: "Oligosaccharide structures of immunoglobulins." Journal of Medical Technology. 39*F. 636-642 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakata, M.: "Rheumatoid factors and oligosaccharide structures." Diagnosis and Treatment. 83. 1173-1177 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsushita, M.: "A novel human serum lectin with collagen-and fibrinogen-like domains which functions as an opsonin." J.Biol.Chem.271. 2448-2454 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "Neoglycolipid as a safe adjuvant for induction of cytotoxic T lymphocyte." AIDS Research Newsletter. 10. 172 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakata, M.: "Significance of galactose-free IgG in rheumatoid arthritis." The Saishin-Igaku. 51. 2354-2359 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Moriwaki, T.: "Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin beta-subunit by disruption of disulfide bonds." Glycoconjugate J.14. 225-229 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "Role of galactosylation in the renal pathogenicity of murine IgG3 monoclonal autoantibodies." Glycoconjugate J.14 Supplement. S138 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakata, M.: "Abnormality in IgG oligosaccharides and activation of complement system by mannose-binding protein." Clinical Immunology. 29. 162-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakata, M.: "Significance of abnormal glycosylation of immunoglobulin G in rheumatoid arthritis." Journal of Clinical and experimental Medicine. 182. 568-572 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shikata, K.: "Structural changes in the oligosaccharide moiety of human IgG with aging." Glycoconjugate J.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizuochi, T.: "The level of galactosylation determines the pathogenicity of murine IgG3 monoclonal autoantibodies." J.Exp.Med.(submitted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sekine, H.: "An ancient, opsonic, lectin-dependent complement system in an ascidian." Nature. (submitted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukasawa, M.: "A new candidate for a safe adjuvant for induction of CD8+ cytotoxic T cells." Int.Immunol.(submitted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tang., W.: "Detection of disease-specific augmentation of abnormal immunoglobulin G in sera of patients with rheumatoid arthritis." Glycoconjugate J.(submitted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakata, M.: The significance of detection of oligosaccharide chains of rheumatoid factor in sera for specific detection of patients with rheumatoid arthritis.Medical Topic Series Ryumachi '95, Medical Review Press, Tokyo, 96-102 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Moriwaki,T.: "Alteration of N-linked oligosacchaude structures of human chouonic gonadotiopin β-subunit by disruption of disulfide bonds." Glycoconjugate J.14. 225-229 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakata,M.: "Effect of aromatic nitroso-compounds on superoxide-generating activity in neutrophils." J.Biochem.122. 188-192 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nasuda,A.-K.: "Mechanisms of nitroso compound induced inhibition of superoxide generation in neutrophils." J.Biochem.122. 550-555 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shikata,K.: "Structural changes in the oligosacchaude moiety of human IgG with aging." Glycoconjugate J.(印刷中). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 中田宗宏: "糖鎖異常IgGとマンノース結合蛋白質による補体系の活性化" 臨床免疫. 29. 162-168 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 中田宗宏: "慢性関節リウマチにおけるIgG糖鎖異常の意義" 医学のあゆみ. 182. 568-572 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 水落 次男: "糖鎖生物学「HIVと糖鎖」" 共立出版(印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushita,M.: "A novel human serum lectin with collagen-and fibrinogen-like domains which functions as an opsonin." J.Biol.Chem.271. 2448-2454 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Moriwaki,T.: "Alteration of N-linked oligosaccharide structures of human chorionic gonadatropin β-subunit by dissuption of disulfide bonds" Glycoconjugate J.印刷中 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 水落次男: "慢性関節リウマチにおけるガラクトース欠損IgGの病因的意義" 最新医学. 51. 2354-2359 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 中田宗宏: "糖鎖異常IgGとマンノース結合蛋白質による補体系の活性化" 臨床免疫. 29. 162-168 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 水落次男: "グライコバイオロジー実験プロトコール" 秀潤社, 394 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugimoto, M.: "Oligomannose-coated liposomes as an adjuvant for the induction of cell-medlated immunity" FEBS Lett.363. 53-56 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsushita, M.: "A novel human serum lectin with callagen-and fibrinogen-like domain which functions as an opsonin" J. Biol. Chem.271. 2448-2454 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 水落次男: "HIV表面糖鎖結合新型エイズ治療薬の開発" 日本臨床. 53. 2340-2349 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中田宗宏: "免疫グロブリンの糖鎖構造" 臨床検査. 39. 636-642 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中田宗宏: "リウマトイド因子と糖鎖構造" 診断と治療. 83. 1173-1177 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 中田宗宏: "Medical Topics Series リウマチ '95" RA診断におけるリウマチ因子糖鎖測定の意義, 96-102 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 志方幸道: "リウマチ病セミナーVI (永井書店)" 慢性関節リウマチにおける糖鎖異常, 184-193 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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