| Project/Area Number |
07557227
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
内科学一般
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
YAMAMOTO Kazuhiko Medical Institute of Bioregulation, Kyushu University, Professor, 生体防御医学研究所, 教授 (80191394)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOKAWA Satoshi Medical Institute of Bioregulation, Kyushu University, Assistant Professor, 生体防御医学研究所, 講師 (20215940)
EZAKI Ichiko Medical Institute of Bioregulation, Kyushu University, Research Associate, 生体防御医学研究所, 助手 (40037453)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | T Cell Receptor / PCR / SSCP / T Cell / Rheumatoid Arthritis / Systemic Lupus Erythematosus |
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| Research Abstract |
To investigate T cell involvement in antigen-specific immune responses, it is important to detect accumulating T cells at a clonal level in vivo.however, thus far the clinical application of sush analyzes has been limeted. Here we have established novel primers to anneal with T cell reseptor (TCR) beta genes of multiple Vbeta families and applied them to reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis to evaluate peripheral T cell clonality of autoimmue disease patients. As a result, the new Vbeta primers could detect accumulating T cell clones in the periphery of healthy individuals and patients. It was revealed that patients with autoimmune diseases, such as systemic lupus erythematosus (SLE) , had a larger number of clonal accumulations of peripheral T cells compared with normal individuals.thus, the RT-PCR-SSCP systemic using the new multifamily Vbeta primers is the first such laboratory examination to detect T cell clonal expantion, and will provide a simple and sensitive tool to aid in the diagnosis and also in the investigation of the pathoqenes of autoimmune diseases.
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