| Project/Area Number |
07557229
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KAWAGUCHI Hideaki HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF LABORATORY MEDICINE,PROFESSOR, 医学部, 教授 (70161297)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Jyoji UNIVERSITY OF TOKYO,FACULTY OF MEDICINE,DEPARTMENT OF CARDIOVASCULAR BIOMECHANIC, 医学部, 助教授 (20159528)
KITABATAKE Akira HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF CARDIOVASCULAR MEDICINE,PRO, 医学部, 教授 (00124769)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | CLONING / RECEPTORS / POLYMERASE CHAIN REACTION / DIFFERENTIAL DISPLAY / IN-GEL PROTEIN KINASE ASSAY / POSITIVE PRESSURE APPARATUS / 機械センサー / 伸展刺激 / 心筋細胞 / ずり応力 / 圧受容体 / 機械刺激 / 心肥大 / 心不全 / イオンチャンネル |
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| Research Abstract |
CELL IS ALIVE UNDER CHEMICAL,LIGHT AND MECHANICAL STIMULI AND EXTRACELLULAR STRESS.BIOLOGICAL PERCEPTION OF MECHANICAL STRESS INCLUDES HEARING,BALANCE,TOUCH SENSATION,DETECTION OF MOVEMENT OF MUSCLE AND TENDON,AND NOTICE OF VOLUME EXPANSION.MECHANICAL STIMULI INCLUDE FRICTION,PRESSURE AND STRETCH.CELLS ADAPT TO THESE STIMULI BY INTRACELLULAR METABOLISM.CHANGE IN ACTIVITY OF ION CHANNELS RESPONDING TO MECHANICAL STRESS IS CLEAR.HOWEVER,IT IS NOT CLEAR THAT ION CHANNELS ARE MECHANOSENSITIVE RECEPTORS THEMSELVES OR NOT.ONE REASON WHY THEY ARE NOT FOUND IS THAT THEY ARE TINY AND SCARCE IN CELLS.IN SPITE OF THIS SITUATION RECENT DEVELOPMENT OF RESEARCH TECHNIQUES IN MOLECULAR BIOLOGY MAKES IT POSSIBLE TO SOLVE THE PROBLEM.OUR STUDY APPLIED SEVERAL STRATE GIES AS FOLLOWS : 1) CLONING OF MECHANOSENSITIVE RECEPTOR cDNAs BY POLYMERASE CHAIN REACTION BASED METHODS.HOWEVER,WE IDENTIFIED cDNAs ALREADY REPORTED ; 2) DEVELOPMENT OF APPARATUS OF POSITIVE PRESSURE ON CULTURE CELLS.THIS APPARATUS IS UNDER USE TO ONGOING STUDY ; 3) IN-GEL PROTEIN KINASE ASSAY METHOD AND 4) mRNA DIFFERENTIAL DISPLAY METHOD ARE UNDER USE TO STUDY CHANGE IN INTRACELLULAR SECOND MESSENGER SYSTEMS AND TO FIND NOVEL GENES WHICH RESPOND TO EXTRACELLULAR POSITIVE PRESSURE.
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