| Project/Area Number |
07557236
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Pediatrics
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| Research Institution | National Institute of Neuroscience, National Center of Neurology nad Psychiatry |
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Principal Investigator |
TAKASHIMA Sachio National Institute of Neuroscience, National Center of Neurology and Psychiatry, Director of Department, 神経研究所2部, 部長 (70038743)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOZAKI Fumihiro Uniquw Medical Co.Director, 部長
許斐 博史 国立精神神経センター, 神経研究所2部, 研究員 (00186719)
TSURU Akira National Institute of Neuroscience, NCNP,Researcher, 神経研究所2部, 研究員
ARAI Yasuhiro National Institute of Neuroscience, NCNP,Researcher, 神経研究所2部, 研究員
KONOMI Hiroshi National Institute of Neuroscience, NCNP,Researcher
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | nitrogen oxide / cerebral circulation / monitoring / 脳血流 / 低酸素症 |
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| Research Abstract |
Electrode method utilizing electrolysis reaction was selected for exploring AMP.This method was thought to be suitable continuously to measure NO gas produced in solution of blood, tissue and liquid. Because half reduction time of NO gas is very short, response speeed was adjusted NO concentration to be 0.05 sec (2Hg). And because very weak concentration has to be measured, noise was prevented in various points. Sensor was 500 mum in size of carbon material and coated special membrane on tip form. As a mambrane, Nafion and silicon were utilized to make thin membrane, and using this membrane, NO measurement was tried in various solutions. NO did not response in thick membrane, but in thin membrane the sensor was well responsed to NO bubling. However, this thin membrane was easily broken by NO bubling, and then silicon mebrane with the drop of TSE399 spread over the surface of the water was coated on the tip, keeping water temperature 21ﾟC degree. Furthermore, because silicon mambrane is fragile, rubber membrane was used. By using nafion and silicon on carbon, relatively stable NO measurement was able to be obtained. However, there were still several problems in membrane stability and calibration of the absolute values. On the other hand, several studies of NOSs were experimentally and histochemically done on their distribution and function, and the significance of their antagonist.
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