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Exploitation of continuous monitoring apparatus of nitric oxide in vivo

Research Project

Project/Area Number 07557236
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Pediatrics
Research InstitutionNational Institute of Neuroscience, National Center of Neurology nad Psychiatry

Principal Investigator

TAKASHIMA Sachio  National Institute of Neuroscience, National Center of Neurology and Psychiatry, Director of Department, 神経研究所2部, 部長 (70038743)

Co-Investigator(Kenkyū-buntansha) SHIOZAKI Fumihiro  Uniquw Medical Co.Director, 部長
許斐 博史  国立精神神経センター, 神経研究所2部, 研究員 (00186719)
TSURU Akira  National Institute of Neuroscience, NCNP,Researcher, 神経研究所2部, 研究員
ARAI Yasuhiro  National Institute of Neuroscience, NCNP,Researcher, 神経研究所2部, 研究員
KONOMI Hiroshi  National Institute of Neuroscience, NCNP,Researcher
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsnitrogen oxide / cerebral circulation / monitoring / 脳血流 / 低酸素症
Research Abstract

Electrode method utilizing electrolysis reaction was selected for exploring AMP.This method was thought to be suitable continuously to measure NO gas produced in solution of blood, tissue and liquid. Because half reduction time of NO gas is very short, response speeed was adjusted NO concentration to be 0.05 sec (2Hg). And because very weak concentration has to be measured, noise was prevented in various points. Sensor was 500 mum in size of carbon material and coated special membrane on tip form. As a mambrane, Nafion and silicon were utilized to make thin membrane, and using this membrane, NO measurement was tried in various solutions. NO did not response in thick membrane, but in thin membrane the sensor was well responsed to NO bubling. However, this thin membrane was easily broken by NO bubling, and then silicon mebrane with the drop of TSE399 spread over the surface of the water was coated on the tip, keeping water temperature 21゚C degree. Furthermore, because silicon mambrane is fragile, rubber membrane was used. By using nafion and silicon on carbon, relatively stable NO measurement was able to be obtained. However, there were still several problems in membrane stability and calibration of the absolute values. On the other hand, several studies of NOSs were experimentally and histochemically done on their distribution and function, and the significance of their antagonist.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report

Research Products

(11 results)

All Other

All Publications (11 results)

  • [Publications] 高嶋 幸男: "硫酸マグネシウムの新生仔脳梗塞形成に対する影響" 日本未熟児新生児学会. 9. 31-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高嶋 幸男: "重症心身障害児・者への呼吸に対する運動療法が脳血液流量へ与える影響" 理学療法学. 24. 389-393 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高嶋 幸男: "幼若家兎てんかんモデルにおける脳循環およびNO動態の変化" 脳と発達. 29. 180 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高嶋 幸男: "過換気における脳内NOと脳血流動態" 脳と発達. 29. S296 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高嶋 幸男: "新生児けいれんモデルの脳循環および脳内NOとグルタミン酸の連続モニター" 日本未熟児新生児学会雑誌. 9. 341 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高嶋 幸男: "神経型nNOS含有神経細胞の発達と周産期脳障害発生への関連" 日本未熟児新生児学会雑誌. 9. 440 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高嶋幸男: "硫酸マグネシウムの新生仔脳梗塞形成に対する影響" 日本未熟児新生児学会. 9. 31-36 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高嶋幸男: "重症心身障害児・者への呼吸に対する運動療法が脳血液流量へ与える影響" 理学療法学. 24. 389-393 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takashima S: "Cerebral hemodynamics on near-infrared spectroscopy in hypoxia and ischemia in young animal studies" Brain and Development,. 17. 312-6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Miyawaki T: "Development of endothelial nitric synthase in endothelial cells in the human cerebrum" Developmental Brain Research,. 89. 161-166 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimoto H: "The effect of acetazolamide and carbon dioxide on cerebral hemodynamic changes on NIRS in young rabbits" Brain and Development,. 17. 261-263 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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