| Project/Area Number |
07557283
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MIKI Mirei TOHOKU UNIV., SCHOOL OF DENT., ASSISTANT PROFESSOR, 歯学部, 助手 (10236820)
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| Co-Investigator(Kenkyū-buntansha) |
MITANI Hideo TOHOKU UNIV., SCHOOL OF DENT., PROFESSOR, 歯学部, 教授 (50014220)
KAGAYAMA Manabu TOHOKU UNIV., SCHOOL OF DENT., PROFESSOR, 歯学部, 教授 (60004610)
SHIMIZU Yoshinobu TOHOKU UNIV., SCHOOL OF DENT., ASSOCIATE PROFESSOR, 歯学部, 助教授 (20005078)
五十嵐 薫 東北大学, 歯学部, 助手 (70202851)
溝口 到 東北大学, 歯学部・付属病院, 講師 (20200032)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Mechanical stress / Signal transduction / Periodontal ligament / Extra cellular matrix / Osteoblasts / Osteoclasts / Cementblasts / Cytoskeleton / 細胞内シグナル伝達 / 歯周組織 / インテグリン / 歯根膜線維芽細胞 / 細胞内骨格 / チロシンリン酸化 / チロシン燐酸化 / 末梢血リンパ球・単球 / 歯根膜細胞 / サイトカイン / 接着分子 / 骨改造 / 歯の移動 |
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| Research Abstract |
1.Osteoblast-like cells, MC3T3-El or periodontal-ligament derived fibroblasts were cultured on Type I collagen-coated silicon membranes and subjected to cyclic mechanical stimulation. The cells assumed a spindle shape, and the cytoskeleton components, including F-actin filaments and tubulin, were aligned perpendicular to the stretch force vector. Tyrosine-phosphorylated molecules which are localized in focal adhesion plaques and MAP kinase might be related to signal transduction caused by mechanical stress.
2.When peripheral blood leukocytes/monocytes were cultured with M-CSF,the number of osteoclast like cells, which were multinuclear, lamellipodia-positive and TRAP-positive cells increased. When these cells were cultured on slices of bovine dental root, pit formations were observed. Macrophages of PBL proliferate by blastogenesis and a part of the blastgenic macrophages differentiate to osteoclasts by M-CSF and 1,25 (OH) _2D_3. Moreover the number of osteoclast-like cells shown in PBL
… More
treated in M-CSF or GM-CSF was suppressed by coculture fluid of PBL/PDL cells.
3.Mechanical stress of tooth movement differently affected the alveolar bone and cellular cementum ; the bone was resorbed whereas the cementum resisted resorption and its calcification is inhibited under the compressive force of tooth movement. The expression of CH-6S is related to the compressive force in non-hyalinized and hyalinized PDL,whereas that of CH-4S/DS is not influenced by the mechanical stress. Osteocalcin expression of cementblasts forming acellular cementum is similar to that of cells forming cellular cementum as well as osteoblasts and odontblasts, and has a role for calcification of acellular cementum. Topical administration of a bisphosphonate (Risedronate) inhibits orthodontic tooth movement and root resorption.
4.Localization and distribution of the ECMs ; Type I,II,III,X collagen, osteocalcin and GAGs were analyzed, using immunohistochemical techniques. The marked regional differences cxit in the phenotypic expression of ECMs in tissues, may reflect the local functional environment and cellular response. Less
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