| Project/Area Number |
07557299
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Physical pharmacy
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| Research Institution | Mukogawa Women's University |
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Principal Investigator |
HAGINAKA Jun Mukogawa Women's Univ., Faculty of Pharm.Sci., Professor, 薬学部, 教授 (20164759)
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| Co-Investigator(Kenkyū-buntansha) |
ANNAKA Masahiko Mitsubishi Chemical Co, Senior Research Scientist, 横浜総研, 研究員
HOSOYA Ken Kyoto Institute of Technology, Associate Professor, 繊維学部, 助教授 (00209248)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | molecular imprinting / glycoconjugate / molecular recognition / HPLC packing material / HPLC用充てん剤 / 認識 / HPLC充填剤 / 糖鎖 / 多段階膨潤重合法 |
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| Research Abstract |
We have obtained the following research results ;
1)Traditional non-covalent type molecular imprinting technique was found to be inapplicable to the molecular imprinting for sugars due to their poor solubility in common organic solvents.
2)We gave developed novel surface imprinting technique for the sugars where template, sugar was added with functional monomers to form new recognition site on the base particles. Those polymer showed specific recognition towards directions of hydroxyl groups at 3,4, and 6 positions of the sugars.
3)We proved that those polymers are able to recognize sugar derivatives in high performance liquid chromatography (HPLC) and much higher recognition ability was observed in adsorption experiment for sugars in aqueous solution.
4)We have developed another molecular imprinting method for sugars. In this method, we prepared polymer having functional group, first. Then, sugars were adsorbed onto the polymer and heat treatment was performed.
5)We utilized methacrylic acid as the functional monomer with ethylene dimethacrylate as cross-linking agents and methanol dissolving a sugar derivative to prepare "pre"-polymer. After the polymerization, and removal of methanol, we applied temperature (up to 200ﾟC for 30 min) to the polymer. Through this heat treatment, specific molecular recognition ability towards sugars was greatly improved.
6)Although traditional molecular imprinting technique was not effective for sugars, newly invented imprinting methods, namely surface imprinting heat imprinting showed much better molecular recognition abilities. These procedures are relatively simple and easy to use.
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