| Project/Area Number |
07557343
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SASAYAMA Shigetake Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (70109007)
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| Co-Investigator(Kenkyū-buntansha) |
KIHARA Yasuki Kyoto University, Graduate School of Medicine, Assistant, 医学研究科, 助手 (40214853)
MATSUMORI Akira Kyoto University, Graduate School of Med, Instructor, 医学研究科, 講師 (70135573)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | oxygen tension / myocardial ischemia / ATP / E-C coupling / mitochondria / 心筋細胞 / 酸素分圧 / 好気的代謝 / ストレス蛋白 / カルシウム / 酵素分圧 / 虚血 / 再灌流 / 分子生物学 |
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| Research Abstract |
The tissue oxygen tension is important determinant of the aerobic metabolism. More closely, the tension gradient between the cell surface and the mitochondria might be the more direct regulator of the ATP turnover. Though it has been assumed, the level of intracellular distribution of the oxygen tension in each cell has not been measured yet. In this study, we focused to develop a new system in which the intracellular oxygen distribution was two-dimensionally visualized in a real-time fashion in an isolated single myocyte. The method was derived from the quantification of the regional phosphorescence queching of a phorphilin derivative during the laser excitation on the confocal microscopic apparatus. We have developed a methodology by which the phorphylin derivative was injected into the individual myocyte without significant cellular damage. The study is currently extended to configure the setting of the laser excitation and the data acquisition of the emitted fluorescence in the confocal microscope and the attached computer-based analyzer. The data might provide the essential information of aerobic as well as anaerobic metabolism and the subcellular roles of the oxygen molecule as a transient signal carrier.
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