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Development of melanogenesis inhibitors available for skin bleaching.

Research Project

Project/Area Number 07557347
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Dermatology
Research InstitutionNagoya University (1997)
Akita University (1995-1996)

Principal Investigator

TOMITA Yasushi  Nagoya Univ.Sch.of Med.Professor, 医学部, 教授 (70108512)

Co-Investigator(Kenkyū-buntansha) ONO Hiroki  Nagoya Univ.Sch.of Med, Research Assistant, 医学部, 助手 (50224275)
YAMADA Katsuhisa  Nagoya University Technoble Life Science laboratry, Senior Reseachar, 研究員
ADACHI Ayumi  Nagoya Univ.Sch.of Med, Lecturer, 医学部, 講師 (00151069)
宮村 佳典  秋田大学, 医学部, 助手 (50272034)
佐藤 俊樹  秋田大学, 医学部, 講師 (40187216)
窪田 卓  秋田大学, 医学部, 助手 (60250875)
新澤 みどり  秋田大学, 医学部, 助手 (00208109)
佐藤 典子  秋田大学, 医学部, 助手 (60205953)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsmelanin / melasma / Oryza sative / rice / tyrosinase
Research Abstract

Our research is to find the compound from natural and farm products for external application to bleach or cure skin pigmentation of the patient with melasma and senile pigmentation.
The inhibitive ability of rice extract on melanogenesis was discovered in our screening system to select untimelanogenic compounds. Whole grains of rice, Oryza sative were pulverized, soaked in water, and extracted over one day at 50゚C.The extract solution was heated and filtered. the extract was enriched using an evaporator, and it was applied on various colum ahromatographies such as Sephadex G-25, MCL Gel and Kiesel Gel. Obtained fraction finally show a single peak in the high power liquid chromatography, and we named it "Isolation A"
Isolation A decreased tyrosinase activity and melanogenesis in B-16 mouse melanoma cells in culture. However, it did not affect the activity of cells such as protein synthesis, which iondicates Isolation A selectively blocked the synthesis of tyrosinase protein.
The extract chemical composition of Isolation A and the relationship between Isolation A and the inhibition process of melanogenesis are not known at present, and therefore more studies are required. We hope that Isolation A will be able to be applied for cosmetic or medical treatment.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] K.Yasumoto 他: "Funcctional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes." J.Biol.Chem.272. 503-509 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Matsunaga 他: "Sequence analysis of the human tyrosinase promoter from a patient with tyrosinase-negative oculocutaneous albinism." Pigment Cell.Res.10. 64-67 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Maeda 他: "Comparison of the melanogenesis in human black amd light brown melanocytes." J.Dermatol.Sci.14. 199-206 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 富田 靖: "尋常性白斑" 臨床科学. 33. 1357-1359 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 富田 靖: "白皮症" 皮膚科の臨床. 39. 1055-1060 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 富田 靖: "白皮症と白斑" 小児科診療. 60. 627-631 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yasumoto, et al.: "Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes." J.Biol Chem. 272. 503-509 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J,Matsunaga, et ai.: "Sequence analysis of the human tyrosinase promoter from a patient with tyrosinase-negative oculocutaneous albinism." Pigment Cell Res. 10. 64-67 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Maeda, et al.: "Comparison of the melanogenesis in human black amd light brown melanocytes." J Dermatol Sci. 14. 199-206 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yasumoto 他: "Funcctional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family genes." J.Biol.Chem.272. 503-509 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Matsunaga 他: "Sequence analysis of the human tyrosinaze promoter from a patient with tyrosinase-negative oculocutaneous albinism." Pigment Cell.Res.10. 64-67 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Maeda 他: "Comparison of the melanogenesis in human black amd light brown melanocytes." J.Dermatol.Sci.14. 199-206 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 富田靖: "尋常生白斑" 臨床科学. 33. 1357-1359 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 富田靖: "白皮症" 皮膚科の臨床. 39. 1055-1060 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 富田靖: "白皮症と白斑" 小児科診療. 60. 627-631 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda et al: "Phospholipase induce melanogenesis in organ-cultured-" Photochem Photobiol. 64. 220-223 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sato et al: "Monoclonal antibody MAT-1 against human tyrosinase……" Pigment Cell Res. 9. 72-76 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masunaga et al: "Localization of laminin-5 in the epidermal basement……" J Histochem Cytochem. 44. 1223-1230 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsunaga et al: "R278TER and P431L mutations of the tyrosinase……" J Dermatol Sci. 13. 134-139 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda et al: "Effect of pituitary and Ovarian hormones on……" Pigment Cell Res. 9. 204-212 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsunaga et al: "Melanogenesis and maliganant melanoma" Elsevier Science B.V., 312 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y Tomita: "The molecular genetics of albinism and piebaldism" Arch Dermatol. 130. 355-358 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] J Matsunaga,et al.: "Detection of point mutation in human tyrosinase gene by improved allele-specific amplification." Exp Dermatol. 4. 377-381 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J Matsunaga, et al.: "R278ER and P431L mutations of tyrosinase gene exist in Japanese patients with tyrosinase-negative oculocutaneous albinism." J Dermatol Sci. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] 富田 靖: "分子生命学的診断" 日皮会誌. 104. 1582-1586 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 富田 靖: "眼皮膚白皮症" 皮膚臨床. 36. 1107-1117 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 富田 靖: "白皮症" 日本小児皮膚科学会雑誌. 13. 59-63 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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