• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Small bowel transplantation using IL-2gamma recepter antibody and trial of rejection monitoring by lipidperoxidation

Research Project

Project/Area Number 07557356
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General surgery
Research InstitutionTohoku University

Principal Investigator

FUNAYAMA Yuji  Tohoku Univ.hospital Lecturer, 医学部附属病院, 講師 (50192315)

Co-Investigator(Kenkyū-buntansha) MASUDA Takayuki  Tohoku Univ.college of Medical Science Professor, 医療技術短期大学部, 教授 (00113910)
NAKAMURA Masataka  Tokyo Med.and Dent.College, Professor., 教授 (30180392)
SASAKI Iwao  Tohoku Univ.School of Med, Associate Professor, 医学部, 助教授 (60125557)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordssmall bowel transplantation / rejection / oxiradical / lipidperoxidation / IL-2 recepter / 脂質過酸化物 / 虚血再環流 / 接着分子 / 活性酸化
Research Abstract

1.Histological evaluation of lymphocyte subsets and adhesion molecules expression *methods* Lymphocyte subsets, ICAM-1 and LFA-1expression in the transplanted small bowel of rats harvested at various periods after transplantation were evaluated immunohistochemically.
*results* In rejected grafts, MHC class II antigen expressed in the early period after transplantation on the proper mucosal layr and crypts. ICAM-1 expressed on the vascular endotherial cells and cell matrix strongly but in the later period, the expression was weakened. LFA-1, which is the antigen expressed on the surface of leukocytes, appeared on the endothelium. Strong staining of LFA-1 observed on the surface of the leukocytes which adhered to the vascular endothelium.
2.Preservation of the small bowel for tlansplantation and the mucosal barrier
*methods* Bacterial translocation was evaluated by measuring blood endotoxin of the rat small bowel transplantation (SBT) models whoes grafts were presreved in Euro-Collin (EC) s … More olution or saline for 1,4 or 8 hours previously to the transplantation.
*results* Endotoxin did not increase in the rats to which small bowel preserved in the EC solution were transplanted whereas the endotoxin level in the rats to which graft preserved in the saline were transplanted elevated. These results suggested that saline may be used for graft preservation if the time from graft harvesting to transplantation is short enouh, but the time must be less than 4 hours.
3.Stimulation of leukocytes by phospholipid peroxide in the SBT
*methods* Mucosal injury was evaluated in the rats to which LTB4 antagonist or allopurinol had been administrated before superior mesenteric artery was occuluded for 30 minites followed by 30 minutes reperfusion.
*results* Both LTB4 antagonist and allopurinol attenuated the injury. But onnly allopurinol supressed PCOOH production. The results suggest that xanthinoxidase rather than leukocytes is a source of free radical and PCOOH may activate leukocyte, rather than be a result of injury. *Summary and Future* Our study proved that in the early phase of graft rejection in small bowel transplantation not only class II antigen but also ICAM-1 is expressed, and leukocytes are activated synchronously. Furtheremore, we demonstrated that PCOOH is a leukocyte activator.
PCOOH may be useful for monitoring small bowel graft rejection, and mucosal injury induced by rejection may be suppress by attenuating PCOOH production. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 増子 毅: "閉塞性黄殖時の急性胃粘膜障害発生の病態について-特に胃粘膜の脂質過酸化の面から" Ulcer Research. 23. 195-197 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 増子 毅: "ラット小腸移植根虚血再灌流障害における脂質過酸化反応の関子" Cytoprotectoor & Biology. 14. 35-37 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masuko et al.: "Pathogenesis of acute gostic mucoral inyury in obstructive jaundiced rat." Ulcer Researd. Vol23.No.2. 195-197 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masuko et al.: "Role of lipid peroxidation in the Pathogenesis of intestinal ischemia-reperfusion injuy of rats" cyto-protection & biology. Vol14.35-37 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 増子 毅: "閉塞性黄疸時の急性胃粘膜障害発生の病態について-とくに胃粘膜の脂質過酸化の面から" Ulcer Research. 23. 195-197 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 増子 毅: "ラット小腸虚血再灌流障害における脂質過酸化反応の関与" Cytoprotectron & Biology. 14. 35-37 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐々木巖: "閉塞性黄疸時の急性胃粘膜障害発生の病態における胃粘膜過酸化脂質とグルタチオンの関" Therapeatic Research. 17. 109-112 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 内藤広郎: "低酸素暴露時の急性胃粘膜病変発生について一特に脂質過酸化の関与について" 登山医学. 15. 91-96 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐々木 巌: "閉塞性黄疸時の急性胃粘膜病変発生の病態における胃粘膜脂質過酸化とグルタチオレの関与について" Therapeutic Research. 17. 109-112 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 増子 毅: "閉塞性黄疸時の急性胃粘膜病変発生の病態について-特に胃粘膜の脂質過酸化の面から" 実験潰瘍. 23. 195-197 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wei-Ming Shian: "gastie muvosal phosplatioeylcbolne Hydvoperooide gncrease During cold Water-Inmersion Restraint stres in Rats." Tohoku J Eop. nud.176. 127-130 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐々木 巌: "急性胃粘膜病変発生における胃粘膜PCOOH" Progressin medicine. 15. 242-245 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宣 偉民: "閉塞性黄疸の急性胃粘膜障害発生における活性酸素の関子に関する実験的検討" Cytoprotection and Biology. 11. 147-150 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宣 偉民: "閉塞性黄疸時の急性胃粘膜病変発生における活性酸素の関与 胃粘膜内TBA反応物質量およびグルタチオン量の変動を中心として" Tberapeutic Research. 15. 77-83 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yuji Funayama: "Cbangis in cell adhesion molecules after small bowel tramsplamtatio in Rats." Trams plantation Proceedings. 27. 595-596 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi