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Development of a new bioartificial liver module

Research Project

Project/Area Number 07557357
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field General surgery
Research InstitutionUniversity of Tokyo

Principal Investigator

MUTO Tetsuichiro  University of Tokyo, Fac of Med, Professor, 医学部・附属病院, 教授 (20110695)

Co-Investigator(Kenkyū-buntansha) SAKAI Yasuyuki  University of Tokyo, Ins of Industrial Science, Assistar, 生産技術研究所, 助手 (00235128)
SUZUKI Motoyuki  University of Tokyo, Ins of Industrial Science, Professo, 生産技術研究所, 教授 (10011040)
NARUSE Katsutoshi  University of Tokyo, Fac of Med, Assistant staff, 医学部・附属病院, 助手
NAGASHIMA Ikuo  University of Tokyo, Fac of Med, Lecturer, 医学部・附属病院, 講師 (90202423)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1996: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsHybrid bioartificial liver / Bioreactor / Polyesten nan-woven fabric / 肝細胞スフェロイド / ブタ肝細胞 / 同種潅流治療 / 同種灌流治療 / 不織布充填型バイオリアクター / ブタ肝細胞スフェロイド / ブタ肝不全モデル
Research Abstract

We devised a bioreactor with high hepatic functional capacity by employing large unmbers of viable hepatocytes and allowing direct contact between the hepatocytes and blood. The newly-developed bioreactor was filled with porcine hepatocytes immobilized on non-woven polyester fabric (NWF) and was given the name NWF bioreactor. Our most recently developed NWF bioreactor is a 200-mL radial-flow-type polycarbonate column, which can accommodate a maximum of 1.3*10^<10> hepatocytes per bioreactor unit. We investigated in vitro efficiency of this bioreactor and obtained promicing results. We utilized a porcine operative liver failure model, and investigated the efficacy of the NWF bioreactor in homologous perfusion conducted to treat pigs with liver failure. Our system of homologous perfusion using a NWF bioreactor has shown promising results in the treatment of pigs with severe liver failure. Our ultimate goal is to treat patients by extracorpoleal homologous perfusion using a NWF bioreactor filled with proliferated human hepatocytes. Our hope is that a hybrid bioartificial liver system incorporating the NWF bioreactor can be used clinically in the future.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Naruse K.,Sakai Y.,Nagashima I.,Jiang GX.,Suzuki M.,Muto T: "Development of a new bioantificical liver module.filled with porene hepatocytes iumobilized on nenwoveu fannc" International Journals of Antificial Organs. 19(6). 347-352 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naruse K,: "Cenpaanisans of percohe hepatocytes to spleroids and sryle hepatocytes in the new-axuein tabvic bioantifieciol liver woelerle" International Journals of Antificial Organs. 19(10). 605-609 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y.: "Lange-scale prepartation and function of parcihe hepatocytes spheroids" International Journals of Antificial Organs. 19(5). 294-306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y.: "Functional stebility of percone hepatocyte spleverds in vantusl anlture sytems cyrdei 100% percure aelb aunerplasua ceudicion" Antificial Organs. 20(1). 56-60 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y.: "Shernt-ternm hypothevnic perservation of porcihe hepatocyte spheverds using VWsdution" Cell Transplantation. 5(4). 505-511 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y.: "In vitro function of parcre hepatocyte spheverds in 100% human plasme" Cell Transplantation. 5(5s-1). S41-S43 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naruse K,Sakai Y,Nagashima I,Jiang GX,Suzuki M,Muto T: "Development of a new bioartificial liver module filled with porcine hepatocytes immobilized on non-woven fabric." Int. J.Artif. Organs. 19 (6). 347-352 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naruse K,Sakai Y,Nagashima I,Jiang GX,Suzuki M,Muto T: "Comparisons of porcine hepatocyte spheroids and single hepatocytes in the non-woven fabric bioartificial liver module." Int. J.Artif. Organs. 19 (10). 605-609 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y,Naruse K,Nagashima I,Muto T,Suzuki M: "Large-scale preparation and function of porcine hepatocyte spheroids" Int. J.Artif. Organs. 19 (5). 294-301 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y,Naruse K,Nagashima I,Muto T,Suzuki M: "Functional stability of porcine hepatocyte spheroids in various culture systems under 100% porcine and human plasma condition." Artif. Organs. 20 (1). 56-60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y,Naruse K,Nagashima I,Muto T,Suzuki M: "Short-term hypothermic preservation of pocine hepatocyte spheroids using UW solution." cell Transplantation. 5 (4). 505-511 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai Y,Naruse K,Nagashima I,Muto T,Suzuki M: "In vitro function of porcine hepatocyte spheroids in 100% human plasma" Cell Transplantation. 5 (5S-1). S41-S43 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naruse K.: "Developmant of a new bioapctificial liven wodrle filled with purcehe hepatoeytes nucerohitired an ven-waven fabric" International Janrnals of Hrtificiel Organs. 19(6). 347-352 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Naruse K.: "Caupeurisons of parcihe hepetceyte splieroids and sirgle Uepatoeytes in the non-woven tabiric biaeutefical ldeu vadul" International Jonrnals of Artificial Organs. 19(10). 605-609 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai Y: "Lauge-scale preqeuration and function of parcihe hepetoeyte spheveride" International Jonrnals of Artificial Organs. 19(5). 294-301 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai Y: "Functional stability of percine hepatocyte spheuoids in voricus culture system under 100% percehe and human plaruer" Antificial Orgeuns. 20(1). 56-60 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai Y: "Shert-tenm hyputhermic preservation of poneve hepetoeyte spharids usay VW solution" Cell Tremsplomtation. 5(4). 505-511 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakai Y: "Invitro function of penere hepertoeyte spleracle in 100% human Pecsrne." Cell Treweplantetion. 5(55-1). 341-343 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 成瀬勝俊.酒井康行: "ブタ肝細胞スフェロイドを固定化した不織布充填型モジュールの性能評価" 人工臓器. 24(3). 821-826 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Naruse. Y. Sakai: "Development of a new bioartificial liuer medule filled with parcine vepatocytes immobilized on uev-aoven fabric" International Journal of Artificial Organs. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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