| Project/Area Number |
07557363
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Urology
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
TOMA Hiroshi Tokyo Women's Medical University, School of Medicine, Dept of Urology, chairman, 医学部・泌尿器科, 教授 (90075549)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Fumio Tokyo Women's Medical University, School of Medicine, Dept of Urology, coadjutor, 医学部・泌尿器科, 助手 (20211683)
ONITSUKA Shiro Tokyo Women's Medical University, School of Medicine, Dept of Urology, coadjutor, 医学部・泌尿器科, 助手 (00204230)
TANABE Kazunari Tokyo Women's Medical University, School of Medicine, Dept of Urology, full-time, 医学部・泌尿器科, 講師 (80188359)
KIHARA Takeshi Tokyo Women's Medical University, School of Medicine, Dept of Urology, full-time, 医学部・泌尿器科, 講師 (60195344)
NAKAZAWA Hayakazu Tokyo Women's Medical University, School of Medicine, Dept of Urology, assisant, 医学部・泌尿器科, 助教授 (00147381)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | gene therapy / renal transplantation / lipsome / acquired cystic disease of the kidney / 体外灌流 / 自家腎移植 / 遺伝子導入 |
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| Research Abstract |
The purpose of this study was to establish the conditions where we can realize the most efficient kidney-specific gene transfer using renal transplantation procedure. The approach to our goal was divided into three steps.
(I) We investigated the effects of temperatures on the efficiency of gene transfer with cationic liposomes into a variety of cells derived from HeLa, LLC-PK I, normal mesangial, and normal endothelial cell lines. At a result, the following became clear : (1) The efficiency of gene transfer with cationic liposomes was almost independent of temperature ; (2) Differences of the efficiency among a variety of cells became smaller with a rise of temperature.
(II) We tried to transfer reporter genes into rat kidneys which was in the convalescent stage from acute tubular necrosis (ATN) and transplanted them orthotopicafly. In advance we had treated rats with HgCl_2 to develop ATN.The kidneys were removed 10 days after transplantation and reporter gene expression was determined using RT-PCR and immunohistochemistry. At a result it was detected in all of three HgCl_2-treated rats and 5 of 6 untreated rats. In addition it was evident in glomerular and interstitial cells, occasionally tubular cells.
(III) We assumed renal cystic disease as a subject of this therapy, then analyzed the molecular basis of cystogenesis and obtained new informations on it.
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