Establishment of the Standard for Radiotherapy on Abnormality of the Suppresser Oncogene p53

Research Project

Project/Area Number	07557367
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	試験
Research Field	病態科学系歯学(含放射線系歯学)
Research Institution	Osaka University
Principal Investigator	FURUKAWA Souhei Osaka University, Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (80173524)
Co-Investigator(Kenkyū-buntansha)	HIRANUMA Hiroko Osaka University, Faculty of Dentistry, clinical staff, 歯学部・附属病院, 医員 FUCHIHATA Hajime Osaka University, Faculty of Dentistry, Professor, 歯学部, 教授 (70028728)
Project Period (FY)	1995 – 1996
Project Status	Completed (Fiscal Year 1996)
Budget Amount *help	¥700,000 (Direct Cost: ¥700,000) Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Keywords	Radiation Therapy / Oncogene p53 / head and neck cancer
Research Abstract	Radiotherapy is an appropriate treatment modality for head and neck cancer, because it seldom is accompanied by functional and aesthetical problem. But in some cases, radiation is not effective. Decision-making is based on analysis of prognostic markers. However, the known factors have a relatively low predictive value. p53 is a tumor suppresser gene and its mutation are considered to be a critical in the development of human malignancies. Since p53 is required for radiation induced apoptosis, analysis of p53 status may be a useful prognostic determinant for head and neck cancer. We analyzed p53 status of patients who were treated at the Department of Oral Radiology, Dental School. Our goal for this study was to determine whether different p53 status had different prognostic implication. 1.Forty-five cases with head and neck cancer were made analysis of p53-oncogene abnormalities by PCR-SSCP method, resulting 18 of 45 (40%) cases gave a positive result. 2.The efficacy of radiotherapy was evaluated with statistical method. The local control rate and the changes or the tumor sizes were estimated with clinical follow-up data and significance was tested by means of X^2. The probability of survival was estimated with Kaplan-Maier method and significance was tested by generalized Wilcoxon test. 3.By multivariate analysis, there were no statistically significant correlation between p53-abnormal cases (positive group) and p53-normal cases (negative group).