| Project/Area Number |
07558078
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Osaka University |
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Principal Investigator |
NOMURA Taisei Osaka University Faculty of Medicine, Professor, 医学部, 教授 (90089871)
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| Co-Investigator(Kenkyū-buntansha) |
KURISHITA Akihiro Procter and Gamble Far East Inc, Technical Center, Toxicology Division, Senior R, アンド・ギャンブル極東(株)・科学技術本部・毒性安全化, 主任研究員
HONGYO Tadashi Osaka University Faculty of Medicine, Assistant Professor, 医学部, 助手 (90271569)
NAKAJIMA Hiroo Osaka University Faculty of Medicine, Assistant Professor, 医学部, 助手 (20237275)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Human tissue in mice / Improved SCID mice / Morphology and function / Human Deseases / Therapeutic model / Thyroid function / Human skin / Solar sensitivity |
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| Research Abstract |
We established an experimental system to investigate the development, morphology, physiology and disorder of human organ and tissue which are maintained in the severe combined immunodeficient mice (SCID) for long period.
1. Long maintenance of normal human tissues and examination of the function.
Normal human organs and tissues (bone marrow, skin, thyroid, colon, stomach, liver, lung, fetal tissues, etc.) were maintained for 2-3 years in the improved SCID mice. These human organs showed normal morphology and function. In particular, thyroid function remained active by the treatment with thyroid stimulating hormone (TSH). Labeled iodine was incorporated into human thyroid gland maintained in the SCID mice more actively by the injection of human TSH to the mice. Human embryonic tissues also well developed and grew rapidly in SCID mice.
2. Experimental toxicology and therapy in human tissues.
Human skin transplanted to the SCID mice were exposed to ultraviolet light B (UVB) for long period. UVB doses more than 5 x 10^5J/m^2 induced actinic keratosis in 75% of transplanted human skin. Human skin which were more sensitives for keratosis induction were also sensitive to UVB for cancer induction. Daily UVB doses more than 2,000 J/m^2 for 2 years (about 2 x 10^6 J/m^2 in total) induced 3 squqamous cell carcinomas in human skin. It was also possible to treat psoriasis and alopecia skin with human lymphocytes.
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