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Research for Image Processing in Protein Crystallography

Research Project

Project/Area Number 07558098
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biophysics
Research InstitutionHimeji Institute of Technology (1996-1997)
The University of Tokushima (1995)

Principal Investigator

MORIMOTO Yukio  Himeji Institute of Technology, 理学部, 助教授 (80200450)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsProtein Crystallography / Image Processing / Image Compress / 画像圧縮 / ウォルッシュ・アダマ-ル変換
Research Abstract

An X-ray protein crystallography requires some diffraction images recorded on imaging-plate, which are extensive data and frames. The size of one data set from one protein crystal is about 65 Mega-byte x 50-100 frames. It is very extensive data volume in a computer disk and/or processing time. This research makes such extensive image data to compress by a new algorithm, fast Fourier transform, and to try to reduce a processing time. The method for a fast transmit of compressed data is also checked by using an optical fiber cable.
An applicant (Y.M.) done the following procedures during a grant delivery period ; (1) a programming of data read/write algorithm, (2) coding the algorithm, (3) an X-ray data acquisition from some protein crytals (proteasome from bovine liver, salicylate hydroxylase, tryptophan synthase, etc) by using a synchrotron radiation, (4) an evaluation of the diffraction image data, (5) a compression of the image data by UNIX computer command and my new algorithms, (6) comparison between two results. A new method is still optimized now. Some of parameters in a cropping image are very important to efficiency of the compression.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 森本 幸生 ほか: "トリプトファン合成酵素αサブユニットの立体構造" 日本生物物理学会予稿集. S9- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 森本 幸生: "X線解析によるプロテアーゼの構造解析" 蛋白質核酸酵素. 42. 2448-2453 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T. Yabuuchi, et al.: "Crystallization and Preliminary X-Ray Analysis of Salicylate Hydroxylase from Pseudomonas putida S-1." J. Biochem.119. 829-831 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 薮内 剛 ほか: "DIP2000/DENZOによるタンパク質結晶のX線回折強度データ収集" 技報. 8. 21-31 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Morimoto, et al.: "The tertiary structure of Tryptophan Synthase alpha-subunit." The proceeding of the Biophysical Society of Japan. S9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Morimoto: "Structural Studies on Proteases by protein Crystallography" PROTEIN,NUCLEIC ACID AND ENZYME. 42. 2448-2453 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yabuuchi, et al.: "Crystallization and Preliminary X-Ray Analysis of Salicylate Hydroxylase from pseudomonas putida S-1." J.Biochem.119. 829-831 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yabuuchi, et al.: "Intensity Data collection from protein crystals by using a DIP2000/DENZO system" GIHOU. 8. 21-31 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 森本幸生: "X線解析によるプロテアーゼの構造解析" 蛋白質核酸酵素. 42. 2448-2453 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Yabuuchi, et.al.: "Crystallization and Preliminary X-Ray Analysis of Salicylate Hydroxylase from Pseudomonas putida S-1" J.Biochem.119. 829-831 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Morimoto, et.al.: "Ordered Structure of the Crystallized Bovine 20S Proteasome" J.Biochem.117. 471-474 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 森本幸生: "球状ウィルスの構造と機能" 生化学. 67. 1236-1240 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 薮内剛ほか: "DIP2000/DENZOによるタンパク質結晶のX線回折強度データ収集" 技報. 8. 21-31 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] T. Yabuuchi: "Crystallization and Preliminary X-Ray Analysis of Salicylate Hydroxylase from Pseudomonas putida S-1." J. Biochem.119. 829-831 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 薮内 剛: "DIP2000/DENZOによるタンパク質結晶のX線回折強度データの収集" 技法. 8. 21-31 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 薮内 剛: "DlP2000/DENZOによるタンパク質結晶のX線回折強度データの収集" 技報. 8. 21-31 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] I.Fujii: "An Integvated X-ray Measurewent and Computalion Systew Cystallogiaphy in Protein" Bioiwages. 2. 143-148 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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