| Project/Area Number |
07558112
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
神経・脳内生理学
|
|---|
| Research Institution | Osaka University (1996-1997)
Okazaki National Research Institutes (1995) |
|---|
Principal Investigator |
KURAHASHI Takashi Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (90225251)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANEKO Akimichi Keio University School of Medicine, Professor, 医学部, 教授 (00051491)
|
|---|
| Project Period (FY) |
1995 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1995: ¥6,400,000 (Direct Cost: ¥6,400,000)
|
|---|
| Keywords | Caged compound / cAMP / ionic channel / Patch clamp / UV flash / Olfactory cell / Retina / computer program / 味細胞 / コンピュータプログラム |
|---|
| Research Abstract |
Olfactory transduction is mediated by the adenylyl cyclase-cAMP system, and their adaptation is regulated by Ca-feedback within the cAMP metabolism. In a qualitative manner, outline of olfactory transduction system is now fairly well understood. However, in the view of quantification, there are still many unresolved questions, since in the field of olfaction research, it is so difficult to control the stimulus delivery In the present study, for response quantification, we aimed to quantify the stimulation to the olfactory receptor cell. To do so, we used caged cAMP and developed a new UV-flash source which can control UV intensity freely and rapidly. Using the whole-cell patch clamp method, we monitored membrane currents sensitive to cytoplasmic cAMP.Photolysis of cytoplasmic caged-cAMP caused rapid activation of cAMP-gated channels. Using this new system, we are planning to quantify the response shape and Ca-dependence of the adaptation in the olfactory receptor cell. In future, the same protocol will be used for the understanding of wide variety of cell functions.
|
