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Construction of hybrid liver support system using gene amplified recombinant mammalian cells

Research Project

Project/Area Number 07558125
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

SUGA Ken-ichi  Faculty of Engineering, Osaka University, Professor, 工学部, 教授 (20029250)

Co-Investigator(Kenkyū-buntansha) OMASA Takeshi  Faculty of Engineering, Osaka University, Assistant Professor, 工学部, 助手 (00252586)
KATAKURA Yoshio  Faculty of Engineering, Osaka University, Assistant Professor, 工学部, 助手 (50263207)
KISHIMOTO Michimasa  Faculty of Engineering, Osaka University, Associate Professor, 工学部, 助教授 (00144436)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1995: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordshybrid artificial liver support system / recombinant DNA / ammonia removal / drug metabolism / gene amplification / glutamine synthetase / 人工肝臓 / 組伝子組換え / 人工肝 / MSX / GS / アンモニア
Research Abstract

In order to establish the cell line which was suitable for hybrid artificial liver support system, an ammonia metabolizing activity was given to Chinese Hamster Ovary (CHO) and HepG2 cell line using genetic engineering techniques. CHO-K1 and HepG2 cells were transformed with pSV2-GS vector which contains glutamine synthetase gene connected with SV40 or CMV promoter. The recombinant cell line was selected under the various concentrations of glutamine synthetase inhibitor, methionine sulfoximine (MSX). The obtained MSX tolerable cell line had the high glutamine synthetase activity and could remove the ammonium from medium. The kinetic parameters of this cell line were determined under 0,1, or 2mM ammonia concentration. The specific ammonium consumption rates of established CHO and HepG2 cell line during growth phase were about one fourth and one seventh of that of primary hepatocyte respectively.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Suga et al.: ""Construction and evaluation of ammonia metabolizing cell line for hybrid bioartificial liver support system using recombinant DNA techniques"" In;"Biochemical Engineeering:Marching toward the Century of Biotechnology"Zhongyao Shen,Fan Ouyang,Juntang Yu and Zhu'an Cao(eds.)Tsinghua University Press,Beijing China. 165-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 菅健 一 ら: ""遺伝子組換えによるハイブリッド型人工肝に適したアンモニア除去細胞の構築の試み"" 化学工学論文集. 24巻2号. 184-189 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suga et al.: ""Construction and evaluation of ammonia metabolizing cell line for hybrid bioartificial liver support system using recombinant DNA techniques"" 化学工学シンポジウムシリーズ. 61. 187-191 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeshi Omasa, Shigeo Urayama, Mitsugu Yamanaka, Naoko Tanimura, Yoshio Katakura, Michimasa Kishimoto, and Ken-ichi Suga: "Construction and evaluation of ammonia metabolizing cell line for hybrid bioartificial liver support system using recombinant DNA techniques" In ; "Biochemical Engineering : Marching toward the Century of Biotechnology" Zhongyao Shen, Fan Ouyang, Juntang Yu and Zhu 'an Cao (eds.) Tsinghua University Press, Beijing China. 165-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeshi Omasa, Naoko Tanimura, Shigeo Urayama, Mitsugu Yamanaka, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga, Shin Enosawa, Hiroshi Amemiya: "Construction of ammonia metabolizing cell line for artificial hybrid liver support system using recombinant DNA techniques" Kagaku kougaku ronbunsyu. vol.24 No.2. 184-189 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeshi Omasa, Naoko Tanimura, Shigeo Urayama, Mitsugu Yamanaka, Yoshio Katakura, Michimasa Kishimoto, and Ken-ichi Suga: "Construction and evaluation of ammonia metabolizing cell line for hybrid bioartificial liver support system using recombinant DNA techniques" "Recent Developments in Biochemical Engineering" Chemical Engineering Symposium series. The Society of Chemical Engineers, Japan. Vol.6. 187-191 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Suga, et al.: ""Construction and evaluation of ammonia metabolizing cell line for hybrid bioartificial liver support system using recombinant DNA techniques"" In;"Biochemical Engineering:Marching toward the Century of Biotechnology"Zhongyao Shen,Fan Ouyang,Juntang Yu and Zhu'an Cao(eds.)Tsinghua University Press,Beijing China. 165-168 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Suga et al: ""Construction and evaluation of ammonia metabolizing cell line for hybrid bioartificial liver support system using recombinant DNA techniques"" 化学工学シンポジウムシリーズ61"Recent Developments in Biochemical Engineering". 61. 187-191 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 菅健一ら: ""遺伝子組換えによるハイブリッド型人工肝に適したアンモニア除去細胞の構築の試み"" 化学工学論文集. 24・2. (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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