| Project/Area Number |
07558134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki College of Allied Health Professions |
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Principal Investigator |
MATSUMOTO Takeshi Kawasaki College of Allied Health Professions, Dept. of Applied Medical Engineering, Assistant Professor, 医用電子技術科, 講師 (30249560)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Takehiko Kyraray Co. Medical Research & Development, Chief Researcher, 開発室, 主任研究員
OGASAWARA Yasuo Kawasaki Medical School, Dept. of Medical Engineering and Systems Cardiology, As, 医学部, 講師 (10152365)
KAJIYA Fumihiko Kawasaki Medical School, Dept. of Medical Engineering and Systems Cardiology, Pr, 医学部, 教授 (70029114)
MOCHIZUKI Seiichi Kawasaki College of Allied Health Professions, Dept. of Applied Medical Engineer, 医用電子技術科, 講師 (60259596)
GOTO Masami Kawasaki College of Allied Health Professions, Dept. of Applied Medical Engineer, 医用電子技術科, 助教授 (50148699)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | coronary vasoregulation / regional myocardial perfusion / nitric oxide / shear flow stress / molecular flow marker / flow heterogeneity / 磁性流体 / 微細NO電極 |
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| Research Abstract |
The intrinsic pattern of myocardial perfusion is determined by coronary vascular tone. Since the most principal sites of coronary vascular tone are the arterioles, the evaluation of coronary circulation on a microscopic level is indispensable to understand the coronary vasoregulatory function. In this study, we applied a newly developed nitric oxide (NO) microsensor to measure the concentration of vascular tissue NO,which plays an essential role in regional myocardial flow regulation. Furthermore, we visualized the spatial pattern of within-layr myocardial perfusion by digital-radiography combined with a molecular deposition technique. The analysis of myocardial perfusion pattern gives valuable information on the coronary vasoregulation because its characteristics of hierarchical structure are reflected in myocardial perfusion pattern. The experimental results were summarized as follows.
The No sensor was inserted into the vascular media of the isolated canine femoral artery, perfused w
… More
ith a Krebs-Henseleit solution. NO concentration in the vascular media increased linearly with perfusion flow rate (flow shear stress on the vascular wall) and also with administration of a substrate of NO synthase, L-arginine. NO production, i. e., measured current was attenuated by perfusing a solution containing an inhibitor of NO synthase, N^<omega>-nitro-L-arginine. It is thus shown that the new NO sensor has satisfactory performance for a selective measurement of NO in the vascular media.
Microregional myocardial flows are more uniform and continuous at high O_2 consuming or low O_2 supply states. There were transmural differences in a regional flow correlation in the left ventricular myocardium, which may be due to the higher O_2 consumption in the subendocardium. Since the regional flow correlation in the subepicardium was increased to a similar level observed in the subendocardium during hypoxia, the subepicardium will allow heterogeneous flow patterns in normoxia, but not anymore in hypoxia in comparison with the subendocardium. Less
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