| Project/Area Number |
07558212
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AKAIKE Toshihiro Dept.of Biomolecular Engineering, Prof., 生命理工学部, 教授 (30101207)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Mitsuaki NeTech Inc., Director, 技術部長(研究職)
MARUYAMA Atsushi Dept.of Biomolecular Engineering, Assis.Prof., 生命理工学部, 助手 (40190566)
WATANABE Yoshifumi Dept.of Biomolecular Engineering, Assis.Prof., 生命理工学部, 助手 (40231013)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥3,600,000 (Direct Cost: ¥3,600,000)
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| Keywords | Liver / Hepatocyte / Biomimetics / beta-Galactose recognition / Asialoglycoprotein model / Drug inclusion / Sinusoidal endothelial / Gene therapy / アシアロ糖タンパク質 / 高分子ミセル / βガラクトース / アシアロ糖タンパク質レセプター / PVLA(ガラクトースポリマー) / ナノパーティクル / β-ガラクトース / アシアロ糖蛋白質 / PVLA / 肝実質細胞 / インテグリンファミリー |
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| Research Abstract |
In drugs and gene delivery, spatio-temporal control of targeting is the most importantsubject by design of cell-recognizable and release-controllable polymeric carriers.
In this research project, oligosaccharide-carrying polymers which are capable of recognizing liver cells and incorporating chemicals and DNA reagents are designed and estimated. It was found out that lactose galactose (1, 4-glucose) -carrying polystyrene derivative (PVLA) is one of the best candidates which have higher capacityof hepatocyte-recognition as well as drugs inclusion by in vitro and in vivo assays.
Poly-lysine, polysaccharide conjugates polymers were also synthesized and characterised as hepatocyte-specific DNA carrier to heptoma cell (HepG2).
Copolymers consisiting of poly-L-lysine backbone and hyaluronic acid side chain were also prepared and evaluated in viewpoint at sinusoidal endothelial cell tergeting as DNA carriers.
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