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Studies on the Biosynthetic Method and Improvement of Production of Novel Polysaccharides from Living Wastes

Research Project

Project/Area Number 07558245
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TOKURA Seiichi  Hokkaido Univ., Graduate School of Envir.Earth Sci., Pro., 大学院地球環境科学研究科, 教授 (40000806)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Tadao  Nihon Soda Co., Ltd., Chief, 機能製品本部, 研究部長
SAKAIRI Nobuo  Hokkaido Univ., Graduate School of Envir.Earth Sci., A.Pro., 大学院地球環境科学研究科, 助教授 (60153863)
NISHI Norio  Hokkaido Univ., Graduate School of Envir.Earth Sci., Pro., 大学院地球環境科学研究科, 教授 (70001857)
白井 昭弘  日本曹達株式会社, 中央研究所, 研究部長
白井 昭宏  日本曹達株式会社, 中央研究所, 研究員
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsBacterial cellulose / Acetobacter xylinum / N-acetylglucosamin / Cultivation / A novel polysaccharides / Direct filamentation / Aerobic rotatory cultivator / Shallow pan cultivator / バクテリアセルロース / グルコース / 静置培養 / 回転通気培養 / アミノ糖残基 / 塩化アンモニウム / 燐酸化アミノ酸 / カルボキシメチルセルロース / N-アセチルグルコサミン残基 / 回転通気培養法 / イオン交換多糖
Research Abstract

Actobacter xylinum has been subcultured repeatedly to prepare N-acetylglucosamin (GlcNAc) residue induced bacterial cellulose (2-4 mole%) applying GlcNAc containing glucose medium. As a similar level of GlcNAc residue incorporation has been made by applying glucosamin or galactosamin as carbonsource instead of GlcNAc, but mannosamin was not effective, participation of deaminase was suggested on the GlcNAc residue incorporation. Since ammonium ion was assumed to be final product by deaminase, gulucose and ammonium salts were applied successfully to incorporation of GlcNAc into bacterial cellulose by applying a new type of aerobic rotatory cultivator. A shallow pan cultivator has been made to prepare fibrous novel bacterial celluloses successfully. These fibers were shown to have higher tensile strength than that of cotton.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Tokura, S., 他3名: "Molecular Weight Dependent Antimicrobial Activity by Chitosan" Macromol.Symp.120. 1-9 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokura, S, 他1名: "Chitin Heparinoids: Their Preparation and Specificities" Functional Monomers and Polymers. 433-454 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, T., 他7名: "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro" Biomaterials. 18. 947-951 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shirai, A., 他3名: "Preparation of a novel(1→4)-β-D-glycan by Acetobacter xylinum--a proposed mechanism for incorporation of a N-acetylglucosamine residue into bacterial cellulose" Carbohydrate Polymers. 32. 223-227 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitamura, H., 他4名: "Marked effect of DNA on collagen fibrillogenesis in vitro" Int.J.Biol.Macromol.20. 241-244 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakairi, N., 他3名: "A Novel Procedure for The Preparation of Fully 6-O-Pivaloylated Derivatives of Cyclodextrin" Carbohydrate Letters. 2. 253-258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokura, S., Ueno, K., Miyazaki, S.and Nishi, N.: "Molecular Weight Dependent Antimicrobial Activity by Chitosan" Macromol.Symp.120. 1-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokura, S.and Nishimura, S.-I.: "Chitin Heparinoids : Their Preparation and Specificities" Functional Monomers and Polymers, Ed.by Takemoto, K., Ottenbrite, R.M., Kamachi, M., Marcel Dekker, Inc.433-454 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori, T., Okumura, M., Matsuura, M., Ueno, K., Tokura, S., Okamoto, Y., Minami, S.and Fujinaga, T.: "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro" Biomaterials. 18. 947-951 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shirai, A., Sakairi, N., Nishi, N.and Tokura, S.: "Preparation of a novel (1*4) -beta-D-glycan by A cetobacter xylinum--a proposed mechanism for incorporation of a N-acetylglucosamine residue into bacterial cellulose" Carbohydrate Polymers. 32. 223-227 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitamura, H., Iwamoto, C., Sakairi, N., Tokura, S., Nishi, N.: "Marked effect of DNA on collagen fibrillogenesis in vitro" Int.J.Biol.Macromol.20. 241-244 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakairi, N., Nomura, K., Nishi, N.and Tokura, S.: "A Novel Procedure for The 'Preparation of Fully 6-O-Pivaloylated Derivatives of Cyclodextrin" Carbohydrate Letters. 2. 253-258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tokura,S., 他3名: "Molecular Weight Dependent Antimicrobial Activity by Chitosan" Macromol.Symp.120. 1-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tokura,S.他1名: "Chitin Heparinoids:Their Preparation and Specificities" Functional Monomers and Polymers. 433-454 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mori,T., 他7名: "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro" Biomaterials. 18. 947-951 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shirai,A., 他3名: "Preparation of a novel (1→4)-β-D-glycan by Acetobacter xylinum--a proposed mechanism for incorporation of a N-acetylglucosamine residue into bacterial-cellulose" Carbohydrate Polymers. 32. 223-227 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitamura,H., 他4名: "Marked effect of DNA on collagen fibrillogenesis in vitro" Int.J.Biol.Macromol.20. 241-244 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakairi,N., 他3名: "A Novel Procedure for The Preparation of Fully 6-O-Pivaloylated Derivatives of Cyclodextrin" Carbohydrate Letters. 2. 253-258 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S. Tokura,他3名: "Biological Activities of Biodegradable Polysaccharide" Macromol. Symp. 101. 389-396 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Inata, S. Tokura他3名: "Utilization of DNA as functional materials : preparation of filters containing DNA insolubilized with alginic acid gel" Int. J. Biol. Mocromol.18. 149-150 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N. Nishikawa, S. Tokura他8名: "Effect of the Substituent Groups of SCM-Chitin-RGDS Conjugate on Inhibitory Activity of Tumor Metastasis" Peptide Chemistry 1995. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Tokura他3名: "Molecular Weight Dependent Antimicrobial by Chitin" New Macromolecular Architecture and Functions. 199-207 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Iwata, S. Tokura他3名: "Study on the Nucleoplasmin-Protamine Interaction in sperm Nuclei" Reports on Progress in Polymer Physics in Japan. 39. 573-576 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H. Kitamura, S. Tokura他3名: "Fibrillogenesis of collagen on DNA-matrix" Nucleic Acids Symposium Series. 35. (245-246)

    • Related Report
      1996 Annual Research Report
  • [Publications] 戸倉清一: "A sulfated chitin,SCM-chitin III, inhibits the clearance of human erythrocytes from the blood circulation in erythrocyte-transfused SCID mice" Immunopharmacology. 29. 65-71 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西 則雄: "SYNTHESIS AND CONFORMATIONAL INVESTIGATION OF TANDEM REPEAT SEQUENCE IN RNA POLYMERASE II" BIO CHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 206. 981-987 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 戸倉清一: "Stabilization of Photo-induced Radecals in Hematoporphyrin-IX-Doped Chitosan Film" Polymer International. 36. 365-372 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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