| Project/Area Number |
07558249
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HAYASHI Toshihiko The University of Tokyo, Graduate School of Arts and Sciences, Professor, 大学院・総合文化研究科, 教授 (60090528)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Kazunori The University of Tokyo, Graduate School of Arts and Sciences, Assistant Profess, 大学院・総合文化研究科, 助手 (70251337)
IMAMURA Yasutada The University of Tokyo, Graduate School of Arts and Sciences, Assistant Profess, 大学院・総合文化研究科, 助手 (40201339)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | COLLAGEN / EXTRACELLULAR MATRIX / TYPE IV COLLAGEN / TYPE IV COLLAGEN GEL / COLLGAEN GEL CULTURE / SMOOTH MUSCLE CELL / ENDOTHELIAL CELL / TYPE V COLLAGEN / I型コラーゲン / マトリジェル / ヒドロキシリジン / 腎メサンジウム細胞 / ゲル / コラーゲンゲル / コラーゲン線維 / 線維芽細胞 / 細胞基質 / 超分子 / 細胞培養 / III型コラーゲン / フィブロネクチン / 多量体形成 |
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| Research Abstract |
The objective of the present study is to elucidate the role of extracellular matrix, collagen protein family in particular, in maintaining the function of multicellular system through a synthetic methodology so that the system may show a novel function. Type I collagen gel culture containing fibroblasts within serves as a dermis-like replacement. We have obtained several bodies of unique information on the effects of type IV collagen gel and type V collagen fibrils on cellular behaviors in culture. The type IV collagen extracted from bovine lens capsule formed gel under unique conditions where specific interactions had been unexpected. Cells, hepatic stellate cells initially, cultured on the type IV collagen gel showed a number of unique behaviors that could never have been seen by the cultures on type I collagen gel or on type IV collagen without a gel form. The cell behaviors included extremely elongated cell morphology, formation of cell-to-cell junction through the tips of cell processes, and repressed proliferation. The observations are shared with other related cells such as smooth muscle cells and glomerular mesangium cells. Endothelial cells, epithelial cells or fibroblasts responded differently. Marker proteins for the contractile stage of smooth muscle cells were expressed when cultured on type IV collagen gel. Reconstituted type V collagen fibrils showed strong inhibition of growth of endothelial cells in spite of the strong cell adhesion.
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