| Project/Area Number |
07558252
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IWATA Hiroo Research Center for Biomedical Engineering Kyoto University, Associate Professor, 生体医療工学研究センター, 助教授 (30160120)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Kazutomo Graduate School of Medicine Kyoto University, Associate Professor, 医学研究科, 助教授 (90168435)
IKADA Yoshito Research Center for Biomedical Engineering Kyoto University, Professor, 生体医療工学研究センター, 教授 (00025909)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Bioartifical Pancreas / islet of Langerhans / Microcapsule / agarose / Complement / Xero-transplantation / pig / dog / 糖尿病 / イヌ / 同種移植 |
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| Research Abstract |
The bioartificial pancreas was designed to incorporate islet tissues and a selectively permeable membrane that isolates islets from the immune system of the recipient. The aim of this study is to examine the possibility of the agarose microcapsule containing allo-islets and xeno-islet as a bioartificial pancreas in canine model.
Allograft : Functional efficacy of the microencapsulated islets was determined using five totally-pancreatectomized diabetic dogs as recipients without immunosuppression. Defined quantity of microencapsulated islets from outbred mongrel donors were grafted through the catheter into omental tissue of the pancreatectomized recipients. In three of five recipients, the average fasting glucose values were controlled under 120 mg/dl for 28,42,49 days without exogenous insulin, which received totally 4.3*103,7.3*103 and 1.0*104 (IE/kg) of microencapsulated islets, respectively.
Xnoe-graft : We have demonstrated that the microcapsule made of an agarose-polystirene sulfonic acid mixture effectively prolonged xeno-islet survival period in rodent models. We supply the agarose and polystirene sulfonic acid to the research group of Nara medical school. They applied our microcapsule to transplantation of porcine islet to canine recipients. The normoglycemic periods of the recipients are 14,42,89, and 99 days.
In conclusion, the present study indicates that the microencapsulated islets can function in large diabetic animals, resulting in the independence of exogenous insulin therapy for prolonged periods without the need for immunosuppression.
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