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A study on human thrombomodulin-immobilized antithrombogenic biomaterials

Research Project

Project/Area Number 07558258
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionKagoshima University

Principal Investigator

AKASHI Mitsuru (1997)  Kagoshima University Faculty of Engineering Professor, 工学部, 教授 (20145460)

岸田 晶夫 (1995-1996)  鹿児島大学, 工学部, 助教授 (60224929)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Shuji  Institute for life science research Asahi Chemical Industry CO., LTD General Man, ライフサイエンス総合研究所・開発薬理研究所, 所長
MARUYAMA Ikuro  Kagoshima University Faculty of Medicine Professor, 医学部, 教授 (20082282)
明石 満  鹿児島大学, 工学部, 教授 (20145460)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Keywordshuman thrombomodulin / immobilization / antithrombogenicity / poly (ethylene) film / dialyzer / cellulose film / affinity interaction / surface plasmon resonance
Research Abstract

Thrombomodulin (TM) is a newly described endothelial cell associated protein that functions as a potentnatural anticoagulant by converting thrombin from a protease to an anticoagulant. Recently, the preparation of reconbinant human thrombomodulin (hTM) was achieved by using gene technology, and since then biomaterials containing hTM have been of interest. In this project, we have done the hTM-immobilization onto various polymer surfaces such as poly (acrylic acid) or poly (vinylamine) grafted poly (ether urethane urea) or poly (ethylene) film using water soluble carbodiimide. As the results, it was apparent that hTM activity was retained enough to show its antithrombogenicity. And then hTM was immobilized onto small dialyzer consisting of cellulose film to carry out blood flow test. Moreover, hTM fragment (E456) was immobilized onto poly (ethylene) film and compared its amount of immobilized, activity and antithrombogenicity with native hTM.With regard to the amount of immobilized, it was apparent that the amount of immobilized of hTM fragment is higher than that of native hTM.Finally, as to compare the activity of native hTM and its fragment, the dissociation constants of f-hTM and thrombin by affinity interaction was evaluated using a surface plasmon resonance.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 岸田 晶夫 他: "ヒト・トロンボモジュリン-フラグメント(E456)の固定化と抗血栓性評価" 第46回高分子学会年次大会、横浜、1997年5月. 46(3). 345- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸田 晶夫 他: "トロンボモジュリンの医用材料への応用" 第7回バイオ・高分子シンポジウム、東京、1997年7月. 71-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸田 晶夫 他: "固定化トロンボモジュリンの医用材料への応用" 日本化学会九州支部・同中国四国支部合同大会、宮崎. 347- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kishida, et al.: "Immobilization of human thrombomodulin fragment (E456) and evaluation of its antithrombogenicity." Polymer Preprint, Japan. 46(3). 345 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kishida, et al.: "Application of human thrombomodulin to medical materials." Proceeding of 7th Biopolymer Symposium. 7172 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kishida, et al.: "Application of the immobilized human thrombomodulin as medical devices." Joint Symposium of Kyushu and Shikoku Branches of Japan Chemical Society. 347 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岸田晶夫 他: "ヒト・トロンボモジュリン-フラグメント(E456)の固定化と抗血栓性評価" 第46回高分子学会年次大会、横浜、1997年5月. 46(3). 345 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岸田晶夫 他: "トロンボモジュリンの医用材料への応用" 第7回バイオ・高分子シンポジウム、東京、1997年7月. 71-72 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岸田晶夫 他: "固定化トロンボモジュリンの医用材料への応用" 日本化学会九州支部・同中国四国支部合同大会、宮崎. 347 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岸田晶夫: "ヒト・トロンボモジュリン固定化材料の合成と抗血栓性評価" 人工臓器. 25・2. 494-497 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akio Kishida: "In Vivo and Vivo Evaluation of the Antithrombogenicity of Human Thrombomodulin Immobilized Biomaterials." ASAIO JournaL. 41. M369-M374 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岸田晶夫: "ヒト・トロンボモジュリン固定化材料の合成と抗血栓性評価" 人工臓器. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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