| Project/Area Number |
07559007
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MIYAKOSHI Junji KYOTO UNIVERSITY,FACULTY OF MEDICINE,ASSOCIATE PROFESSOR, 医学研究科, 助教授 (70121572)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEBE Hiraku KYOTO UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学研究科, 教授 (10028318)
HIRAOKA Masahiro KYOTO UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学研究科, 教授 (70173218)
TATSUMI Junko KYOTO UNIVERSITY,FACULTY OF MEDICINE,RESEARCH ASSOCIATE, 医学研究科, 助手 (80128222)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1995: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | Magnetic fields / Cultured cells / Cell growth / Gene expression / Signal transduction / Mutation induction |
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| Research Abstract |
We had manufactured an appatus for exposure of cultured cells to low density of low frequency magnetic fields. We disigned and manufactured equipments for long-term and low-density (0 to 9 mT) exposures of cultured cells to extremely low frequency magnetic fields (ELFMF), and examined the effects of ELFMF on cell growth, c-myc mRNA expression and the mutation frequency in Chinese hamster ovary (CHO) cells. The ELFMF equipment consists of a CO2 incubator with a built-in magnet generator using Helmholtz coils being 250 mm in inner diameter, 160 mm in distance and 128 turns, a slide regulator and a thermocontroller. No significant difference in the growth rate and the c-mycexpression of CHO cells was observed with 5 mT ELFMF exposure, sham-exposure and incubation in a conventional incubator. On the other hand, CHO-K1 cells were exposed to 5 mT ELFMF at 60 Hz with or without initial X-irradiation for up to 12 weeks, and mutation frequency in the hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene was analyzed using the 6-thioguanine-resistant mutation assay. The long-term exposures for up to 12 weeks to 5mT ELFMF did not increase mutations of HPRT gene in CHO-K1 cells. However, the enhancement of X-ray-induced mutations was observed in the treatment of X-irradiation followed by long-term 5 mT ELFMF exposures. The density of environmental ELFMF from high voltage power-lines should be at least several hundred times less than the density used in this study. Unless there are unknown mechanisms that involve mutagenesis under exposure to very highdensity ELFMF,cancer induction due to environmental ELFMF may be very low or unlikely. However, the effects of the combined exposure to X-rays abd ELFMF should be further investigated.
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