| Project/Area Number |
07559010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
HAKOSHIMA Toshio Nara Institute of Science and Technology Department of Molecular Biology, Professor, バイオサイエンス研究科, 教授 (00164773)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHI Tsuneyuki RIGAKU Co.Ltd.Department of Research and Development, Executive Director Researc, 研究開発部, 部長研究員
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1995: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | imaging palate / X-ray detector / X-ray optics / protein crystallography / molecular / molecular complex / molecular recognition / structural biology / 高輝度 / 結晶 / X線 / データ収集法 / X線発生装置 / 回転対陰極 / 巨大複合体 / X線結晶学 / 生物物理学 |
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| Research Abstract |
An instrument system for X-ray intensity data collection from bio-macromolecular crystals in a laboratory was developed. The system consists of a new X-ray detector (RAXIS-IV) and an improved fine focused (0.1 mm x 1.0 mm) X-ray generator (FR-C). Using two large (300 mm x 300 mm) imaging plates, the detector was designed to yield a short read-out time with high sensitivity. The oil and vacuum sealing of the X-ray generator was improved to yield long-lasting seals. The X-ray beam was focused with Supper's double-mirror. The system was used for data collections of several protein-DNA complex, protein-GTP complex and protein crystals and structure determinations of these crystals were performed using the intensity data obtained. These are the budding yeast transcription factor PHO4 complexed with DNA,the mouse transcription factor IRF-2 complexed with DNA,the fission yeast transcription factor Pap1 complexed with DNA,mouse hippocampal serine protease neuropsin, the human small G protein RhoA complexed with GTPgammaS,the human small G protein RhoA complexed with GTPgammaS,the HPt domain of a E.coli sensor kinase ArcB complexed with a E.coli response regulator CheY,the HPt domain of ArcB a E.coli phospho-histidine phosphatase SixA.
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