THE TRINUCLEAR COPPER CLUSTER IN MULTICOPPER OXIDASES
| Project/Area Number |
07640740
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Inorganic chemistry
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| Research Institution | (INSTITUTE FOR MOLECULAR SCIENCE) Okazaki National Research Institutes (1996)
Kanazawa University (1995) |
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Principal Investigator |
SAKURAI Takeshi INSTITUTE FOR MOLECULAR SCIENCE,ASSOCIATE PROFESSOR, 分子科学研究所, 助教授 (90116038)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | MULTICOPPER OXIDASE / ASCORBATE OXIDASE / LACCASE / TRINUCLEAR CENTER / ESR / SQUID / ANTIFERROMAGNETIC INTERACTION / アスコルビン酸オキシダーゼ / 磁気モーメント / 三核銅クラスター / 酸化還元 / 電子移動 / 電気化学 / 阻害剤 |
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| Research Abstract |
EPR SPECTRA OF LACCASE AND ASCORBATE OXIDASE WERE MEASURED FROM 3 K TO 300 K AND WERE SIMULATED,SHOWING THAT THE STRUCTURE OF TYPE 2 COPPER CHANGES FROM TETRAGONAL AT LOW TENPERATURE TO TETRAHEDRAL AT HIGH TEMPERATURE BECAUSE OF THE PROTEIN CONFORMATION CHANGE.SQUID WAS ALSO MEASURED FROM 5 K TO 300 K AND THE - 2J VALUE OF THE ANTIFERROMAGNETIC INTERACTION BETWEEN TYPE 3 COPPERS WERE DETERMINED AND WAS FOUND THAT THE TRIPLET STATE IS NOT NEGLIGIBLE AT ROOM TEMPERATURE IN THE CASE OF ASCORBATE OXIDASE.THE CAREFUL INSPECTION OF SQUID,ESR SPECTRA AND RESONANCE RAMAN SPECTRA OF THE INCREASING AMOUNT OF AZIDE ACTION ON LACCASE INDICATED THAT AZIDE IS BOUND TO ONE OF TYPE 3 COPPERS WITH THE END-ON FASHION BY WEAKENING THE INTERACTION IN THE TRINUCLEAR CENTER AND FINALLY BREAKING THE INTERACTION BY EXPELLING THE BRIDGING HYDROXIDE FROM COORDINATION SPHERE.
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Report
(3 results)
Research Products
(21 results)
