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THE TRINUCLEAR COPPER CLUSTER IN MULTICOPPER OXIDASES

Research Project

Project/Area Number 07640740
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Inorganic chemistry
Research Institution(INSTITUTE FOR MOLECULAR SCIENCE) Okazaki National Research Institutes (1996)
Kanazawa University (1995)

Principal Investigator

SAKURAI Takeshi  INSTITUTE FOR MOLECULAR SCIENCE,ASSOCIATE PROFESSOR, 分子科学研究所, 助教授 (90116038)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsMULTICOPPER OXIDASE / ASCORBATE OXIDASE / LACCASE / TRINUCLEAR CENTER / ESR / SQUID / ANTIFERROMAGNETIC INTERACTION / アスコルビン酸オキシダーゼ / 磁気モーメント / 三核銅クラスター / 酸化還元 / 電子移動 / 電気化学 / 阻害剤
Research Abstract

EPR SPECTRA OF LACCASE AND ASCORBATE OXIDASE WERE MEASURED FROM 3 K TO 300 K AND WERE SIMULATED,SHOWING THAT THE STRUCTURE OF TYPE 2 COPPER CHANGES FROM TETRAGONAL AT LOW TENPERATURE TO TETRAHEDRAL AT HIGH TEMPERATURE BECAUSE OF THE PROTEIN CONFORMATION CHANGE.SQUID WAS ALSO MEASURED FROM 5 K TO 300 K AND THE - 2J VALUE OF THE ANTIFERROMAGNETIC INTERACTION BETWEEN TYPE 3 COPPERS WERE DETERMINED AND WAS FOUND THAT THE TRIPLET STATE IS NOT NEGLIGIBLE AT ROOM TEMPERATURE IN THE CASE OF ASCORBATE OXIDASE.THE CAREFUL INSPECTION OF SQUID,ESR SPECTRA AND RESONANCE RAMAN SPECTRA OF THE INCREASING AMOUNT OF AZIDE ACTION ON LACCASE INDICATED THAT AZIDE IS BOUND TO ONE OF TYPE 3 COPPERS WITH THE END-ON FASHION BY WEAKENING THE INTERACTION IN THE TRINUCLEAR CENTER AND FINALLY BREAKING THE INTERACTION BY EXPELLING THE BRIDGING HYDROXIDE FROM COORDINATION SPHERE.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 櫻井武 他1名: "Direct Electrochemistry of Blue Copper Proteins at Au Electrodes Modified with Promoters" Chemistry Letters. 1995・8. 1075-1076

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻井武 他1名: "Room temperature ESR Spectra of Rhus vernicifera Laccase and Derivatives" Biochemical and Biophysical Research Communications. 215・1. 235-240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻井武 他1名: "EPR Spectra of Type 3 Copper Centers in Rhus vernicifera Laccase and Cucumis sativus Ascorbate Oxidase" Biochimica et Biophysica Acta. 1248・2. 143-148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻井武: "Cyclic Voltammetry of Cucumber Ascorbate Oxldase" Chemistry Letters. 1996・4. 481-482

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻井武 他2名: "FT-IR Spectra of the Azide-Type 3 Copper in Laccase and Ascorbate Oxidase" Chemistry Letters. 1996・6. 651-652

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻井武 他3名: "Reduction and Oxidation Processes of Blue Copper Proteins,Azurin,Pseudoazurin,Umecyanin,Stellacyanin,Plantacyanin,and Plastocyanin Approached by Cyclic and Potential Step Voltammetries" Bulletin of the Chemical Society of Japan. 69・10. 2855-2862

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKESHI SAKURAI: "DIRECT ELECTROCHEMISTRY OF BLUE COPPER PROTEINS AT AU ELECTRODES MODIFIED WITH PROMOTERS" CHEM.LETT.1075-1076 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKESHI SAKURAI: "ROOM TEMPERATURE ESR SPECTRA OF RHUS VERNICIFERA LACCASE AND DERIVATIVES" BIOCHEM.BIOPHYS.RES.COMMUN.215. 235-240 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKESHI SAKURAI: "EPR SPECTRA OF TYPE 3 COPPER CENTERS IN RHUS VERNICIFERA LACCASE AND CUCUMIS SATIVUS ASCORBATE OXIDASE" BIOCHIM.BIOPHYS.ACTA. 1248. 143-148 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKESHI SAKURAI: "CYCLIC VOLTAMMETRY OF CUCUMBER ASCORBATE OXIDASE" CHEM.LETT.481-482 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKESHI SAKURAI: "FT-IR SPECTRA OF THE AZIDE TYPE 3 COPPER IN LACCASE AND ASCORBATE OXIDASE" CHEM.LETT.651-652 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKESHI SAKURAI: "REDUCTION AND OXIDATION PROCESS OF BLUE COPPER PROTEINS,AZURIN,PSEUDOAZURIN,UMECYANIN,STELLACYANIN,PLANTACYANIN,AND PLASTOCYANIN APPROACHED BY CYCLIC AND POTENTIAL STEP" BULL.CHEM.SCC.JPN.2855-2862 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻井武: "Cyclic Voltammetry of Cucumber Ascorbate Oxidase" Chemistry Letters. 1996・4. 481-482 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 櫻井武 他2名: "FT-IR Spectra of the Azide-Type 3 Copper in Laccase and Ascorbate Oxidase" Chemistry Letters. 1996・6. 651-652 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 櫻井武 他3名: "Reduction and Oxidation Processes of Blue Copper Proteins,Azurin,Pseudoazurin,Umecyanin,Stellacyanin,Plantacyanin,and Plastocyanin Approached by Cyclic and Potential Step Voltammetries" Bulletin of the Chemical Society of Japan. 69・10. 2855-2862 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 櫻井武: "Direct Electrochemistry of Blue Copper Proteins at Au Electrode Modified with Promoters" Chem. Lett.1075-1076 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 櫻井武: "Room Temperature ESR Spectra of Rhus vernicifera Laccase and Derivatives" Biochem. Biophys. Res. Commun.215. 235-240 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 櫻井武: "EPR Spectra of Type 3 Copper Centers in Rhus vernicifera Laccase and Cucumis sativus Ascorbate Oxidase" Biochim. Biophys. Acta. 1248. 143-148 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 櫻井武: ":Restrained Reduction of Type 3 Coppers in Laccase and Ascorbate Oxidase by Azide" Biochem. (Life Sci. Adv. ). 14. 17-21 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 櫻井武: "Spectroscopy of Cucumber Ascorbate Oxidase and Fungal Laccase" World Scientific Publishing Co Pte Ltd, (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 櫻井武: "バイオサイエンスESR(下)" 廣川書店, (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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