| Project/Area Number |
07640810
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
分離・精製・検出法
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MOTONAKA Junko Facalty of Engineering, The University of Tokushima, Associate Professor, 工学部, 助教授 (40035811)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | L-amino acid / D-amino acid / Micro-enzyme sensors / optical isomers / 光学異性体 |
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| Research Abstract |
Micro-enzyme sensors using L-amino-acid oxidase (EC 1.4.3.2) and D-amino-acid oxidase (EC 1.4.3.3) were developed for measuring L- and D-amino acids. The platinum micro-sensors (platinum diameter, 30 mum) were etched in hot aqua regia to create a cavity at their tip. A porous composite material prepared from acetylene black and Teflon emulsion was packed into the tip of the platinum micro-sensors and the redox mediator [Os (bpy)_3 ] (PF_6)_2 was monitored by cyclic voltammetry in the potential range 200-900 mV.The micro-sensors were dipped overnight in buffer solution containing the desired enzyme to immobilize in on the tip by adsorption method. Calibration curves for measurements of L- and D-amino acids, the effects of pH,temperature, and concomitant compounds, the life-time of the micro-sensors, and their availability for measuring L-amino acids in urine were examined. Under optimal conditions, the response of sensors was linear in the concentration ranges of 3.3-240mg/l L-amino acids and 0.83-17mg/l D-amino acids.
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