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Studies on autophagy in tobacco cultured cells

Research Project

Project/Area Number 07640867
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionUniversity of Shizuoka

Principal Investigator

MORIYASU Yuji  University of Shizuoka, School of Food and Nutiritional Sciences, Joshu, 食品栄養科学部, 助手 (20200454)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsautophagy / vacuole / lysosome / protease / autolysis / tobacco / cultured cells
Research Abstract

The plant central vacuole has been thought to be an organelle homologous to animal lysosomes ; it is acidic and contains various kinds of hydrolytic enzymes. However, we do not know if it functions as a proteolytic compartment as animal lysosomes do. To investigate this question, we have studied the mechanism of autophagy induced upon sucrose starvation, using heterotrophically growing tobacco suspension culture cells.
When the cells are incubated in sucrose-free culture medium, bulk degradation of intracellular protein is induced. This induction of protein degradation is inhibited by cysteine protease inhibitors such as E-64, which also cause accumulation of many membrane-bound vesicles in the perinuclear cytoplasm. These vesicles are 1 to 6 micrometers in diameter and have several osmiophilic particles within them. They are acidic compartments and contain acid phosphatase.
These results suggest that the vesicles are plant autolysosomes and that they contribute to cellular protein degradation which is induced by sucrose starvation. Thus plant cells are likely to possess a lytic organelle separate from the central vacuole.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Yuji Moriyasu: "Autophagy in tobacco suspension-cultured cells in response to sucrose starvation" Plant Physiology. 111. 1233-1241 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Moriyasu, Y.and Ohsumi, Y.: "Autophagy in tobacco suspension-cultured cells in response to sucrose starvation." Plant Physiol.111. 1233-1241 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuji Moriyasu: "Autophagy in Tobacco Suspension-Cultured Cells in Response to Sucrose Sttufion" Plant Physiology. 111. 1233-1241 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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