• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Characterization of a plant gene encoding a small GTP-binding protein that is specifically induced by SO_2 and NO_2

Research Project

Project/Area Number 07640875
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionBiotechnology Institute, Akita Prefectural College of Agriculture

Principal Investigator

YOUSSEFIAN Shohab  Akita Pref.Coll.Agric., Biotech.Inst., Assoc Profess, 附属生物工学研究所, 助教授 (00210576)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Keywordswheat / GTP / small G protein / oxidative stress / sulphur dioxide / nitrogen dioxide / transgenic plants / accessory proteins / Wgp
Research Abstract

The ras-related small GTP-binding proteins have been implicated in the regulation of various cellular processes including growth, differentiation, cytoskeletal organization, intracellular vesicle transport and secretion.Such genes have been identified in plants but little is known of the mechanisms by which they are regulated or the functins of their encoded proteins.The wheat gene, wgp7, encoding a small GTP-binding protein, was first identified because of its increased transcript levels by SO_2 and NO_2.Over the last 3 years, the following results have been obtained.
1.The wgp7 transcripts are rapidly induced not only by SO_2 and NO_2, in a light-dependant manner, but also by conditions that specifically induce oxidative stress, including the herbicide methyl viologen (paraquat), a catalase inhibitor, and by exposure to cold or UV-C.
2.Transgenic tobacco plants (including T2 and T3 generations) were produced with the wgp7 gene in a sense orientation under control of the CaMV 35S promot … More er.Depending on the level of transgene expression, plants with differing morphologies were obtained, including a severe rosette dwarf.High levels of wgp7 transcripts and proteins were expressed in these plants, but no clear differences in response to several oxidative stress conditions have yet been identified.
3.The promoter region of wgp7 has been cloned, and partially sequenced.Current studies are aimed to identify the cis-elements essential for oxidative stress regulated expression.
4.Rocombinant wgp7 protein, synthesized in vitro, was shown to bind GTP.The full length and also highly-variable C-terminal end of the protein were used to raise polyclonal antibodies.Subcellular fractionation of proteins from transgenic tobacco showed that the wgp7 protein was localized to the microsomal fraction, suggesting that it is membrane-bound.In wheat, the wgp7 protein was rapidly induced by oxidative stress treatment.Experiments for subcellular localization of the protein following oxidative stress are underway.
5.Using the wgp7 protein, as well as another small GTP-binding protein, interaction cloning was used to isolate interacting proteins.Consequently, a cDNA clone, encoding a novel protein was identified.Binding of this protein was found to be specific, reversible and priferentially with the small GTP-binding protein in its GDP-bound form.Deletion analysis of this protein showed that an N-terminal specific region of 198 amino acids is sufficient for binding the small GTP-binding protein. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Sano H.& Youssefian S.: "Light and nutritional regulation of transcripts encoding a novel protein kinase from wheat is mediated by cytokinins" Proc.Natl.Acad.Sci.91. 2582-2586 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sano, Seo, Orudgev, Youssefian Ishizuka, Ohashi,: "Expression of asmall GTP-binding protein in tobacco plants elevates cytokinin levels,abnormally incdues salicylic acid in response to wounding,and increases resistance to tobacco mosaic virus infection" Proc.Natl.Acad.Sci.91. 10556-10560 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Youssefian, S., Nakamura, M., and Sano, H.: "Molecular characterization of rgp2 a gene encoding a small GTP-binding protein from rice." Mol.Gen.Genet.237. 187-192 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kamada, I., Yamauchi S., Youssefian, S.and Sano, H.: "Transgenic tobacco plants expressing rgp1 a gene encoding a ras-related GTP-binding protein from rice,show distinct morphological characteristics." The Plant Journal. 2. 799-807 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sano, H.and Youssefian, S.: "A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice." Mol.Gen.Genet.228. 227-232 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sano, H., Seo, S., Orudgev, E., Youssefian, S., Ishizuka, K.and Ohashi, Y.: "Expression of a small GTP-binding protein in tobacco plants elevates cytokinin levels and abnormally induces salicylic acid in response to wounding and increases resistance to tobacco mosaic virus infection" Proc.Natl.Acad.Sci.91. 10556-10560 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sano, H., Youssefian, S: "Light and nutritional regulation of transcripts encoding a novel protein kinase from wheat is mediated by cytokinins." Proc.Natl.Acad.Sci.91. 2582-2586 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Youssefian, S., Nakamura, M., and Sano, H.: "Molecular characterization of rgp2, a gene encoding a small GTP-binding protein from rice." Mol.Gen.Genet.237. 187-192 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Youssefian, S., Nakamura, M., and Sano, H.: "Tobacco plants transformed with the O-acetylserine (thiol) lyase gene of wheat are resistant to toxic levels of hydrogen sulphide gas." The Plant Journal. 4 (5). 759-769 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kamada, I., Yamauchi S., Youssefian, S.and Sano, H.: "Transgenic tobacco plants expressing rgp1, a gene encoding a ras-related GTP-binding protein from rice, show distinct morphological characteristics." The Plant Journal. 2. 799-807 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sano, H.and Youssefian, S.: "A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice." Mol.Gen.Genet.228. 227-232 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sano H.& Youssefian S.: "Light and nutritional regulation of transcripts encoding a novel protein kinase from wheat is mediated by cytokinins" Proc.Natl.Acad.Sci. 91. 2582-2586 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sano,Seo,Orudgev,Youssefian Ishizuka,Ohashi,: "Expression of a small GTP-binding protein in tobacco plants elevates cytokinin levels,abnormally induces salicylic acid in response to wounding,and increses resistance to tobacco mosaic virus infection" Proc.Natl.Acad.Sci.91. 10556-10560 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Youssefian,S,Nakamura,M.,and Sano,H.: "Molecular characterization of rgp2,a gene encoding a small GTP-binding protein from rice." Mol.Gen.Genet.237. 187-192 (1993)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kamada,I.,Yamauchi S.,Youssefian,S.and Sano,H.: "Transgenic tobacco plants expressing rgp1,a gene encoding a ras-related GTP-binding protein from rice,show distinct morphological characterisitcs." The Plant Journal. 2. 799-807 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sano,H.and Youssefians,S.: "A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice." Mol.Gen.Genet.228. 227-232 (1991)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sano H.& Youssefian S.: "Light and nutritional regulation of transcripts encoding a novel protein kinase from wheat is mediated by cytokinins" Proc. Natl. Acad. Sci.91. 2582-2586 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sano,Seo, Orudgev,Youssefian Ishizuka,Ohashi,: "Expression of a small GTP-binding protein in tobacco plants elevates cytokinin levels,abnormally induces salicylic acid in response to wounding,and increases resistance to tobacco mosaic virus infection" Proc. Natl. Acad. Sci.91. 10556-10560 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Youssefian,S.,Nakamura,M.,and Sano,H.: "Molecular characterization of rgp2,a gene encoding a small GTP-binding protein from rice." Mol. Gen. Genet.237. 187-192 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kamada,I.,Yamauchi S.,Youssefian,S.and Sano,H.: "Transgenic tobacco plants expressing rgp1,a gene encoding a ras-related GTP-binding protein from rice,show distinct morphological characteristics." The Plant Journal. 2. 799-807 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sano,H.and Youssefian,S.: "A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice." Mol. Gen. Genet.228. 227-232 (1991)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi