| Project/Area Number |
07640876
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | The University of Tokyo (1996)
National Institute of Genetics (1995) |
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Principal Investigator |
HIROYUKI Hirano The University of Tokyo, Graduate School of Agricultural and Life Science, Asso. Prof., 大学院・農学生命科学研究科, 助教授 (00192716)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | starch / Wx gene / rice (Oryza sativa) / cloning / gene expression / pericarp / イネ / 遺伝子 / wx遺伝子座 |
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| Research Abstract |
The rice wx locus controls amylose synthesis in the endosperm and pollen. We prepared that antiserum raised against the Wx protein and used for the immunoblot analysis using protein extract from various tissue. We found a clear band in the extract from developing seeds in quite early stage (3-4 days after anthesis). This protein band was detected in the protein extract from the same tissue of wx strains, suggesting that this protein, designated as WRP1 (Wx protein-related protein 1), was different from the gene product of the wx locus. The further analysis indicated that the WRP1 was expressed in the pericarp, where photosynthesis actively occurred in the early stage of seed development. As the of green color (an indication of photosynthetic reaction) in pericarp tissues became pale, the expression level of WRP gradually decreased. Therefore, we presumed that WRP1 might have partial similarity to Wx protein and catalyze the synthesis of assimilation starch. Thus, as the first step to isolate the gene responsible for the synthesis of assimilation starch, we tried to clone cDNA for WRP1 and obtained several candidates. Preliminary results showed that one clone strongly expressed in the pericarp and its temporal expression pattern was similar to that of WRP1. We would like to confirm these results and to advance our research to isolate the gene for the assimilation starch synthesis.
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