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The regulatory mechanism of endothelin on the secretion of the pituitary horones

Research Project

Project/Area Number 07640888
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionKanagawa Dental College

Principal Investigator

UEMURA Haruko  Kanagawa Dent.Coll., Fac.Dent., Professor, 歯学部, 教授 (00147985)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Toshiharu  Kanagawa Dent.Coll., Fac.Dent., Lecturer, 歯学部, 講師 (50111901)
中村 澄夫  神奈川歯科大学, 歯学部, 講師 (80104496)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsendothelin / endothelin receptor / neurohypophysis / gonadotroph / adenohypophysis / vasopressin
Research Abstract

1. (1) The distribution of endothelin-3 (ET3)-like immunoreactivity in the bullfrog pituitary was investigated immunohistochemically. ET3-immunoreactive (ET3-ir) cells were found to correspond to cells showing LH- and FSH -immunoreactivities. In the male pituitary, only a few ET3-ir cells were observed. (2) Immuno-electron microscopic analysis using colloidal gold revealed the co-existence of ET3-like substance (s) and gonadotropins within the same secretory granules. These results suggest the possible participation of ET3 in regulating pituitary function as an autocrine and/or paractrine hormone.
2. Antisera were raised against amino-acid residues 425-439 of the rat ET B receptor (ET_B receptor). Massive ET_B receptor immunoreactive fibers were observed in the median eminence and organum vasculosum lamina terminalis of rats. Double labeling studies with antiluteinzing hormone-releasing hormone (LHRH) antiserum revealed that ET_B receptor-like immunoreactivity associated with LHRH-immunoreactive fibers in these areas. However, ET_B receptor-like immunoreactivity was not detected in LHRH-immunoreactive somata. This result suggests that ET affects the LHRH neuronal systems via ET_B receptors on the axon at the terminal field.
3. Effects of ET-1 on the release of vasopressin (VP) from the isolated neural lobe of the rat were investigated. In addition, effects of angiotensin II (ANG II) were also investigated, because it colocalizes with ET-1 and VP in the same secretory granules in the axon terminals. ET-1 induced an increase in VP release, followed by a decline to basal levels upon its withdrawal. The VP release was also stimulated by ANG II.These results strongly suggest that ET-1 and ANG II act directly on the neural lobe to stimulate the release of VP.However, the sites of action of these peptides are not clear.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Yamamoto,Toshiharu: "Differential distributions of endothelin A receptor-like and endothelin B receptor-like immunoreactivities in the rat median eminence." Zoological Science. 12(Suppl). 9- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山本利春: "ラット視床下部におけるエンドセリン受容体の分布(免疫組織化学による検索)" 日本内分泌学会雑誌. 71. 810- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,Hirohumi: "Distribution of endothelin 3-like immunoreactivity in the gonadotroph of the female bullfrog." Zoological Science. 13(Suppl). 12- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki Hirohumi: "Distribution of endothelin 3-like immunoreactivity in gonadotrophs of the bullfrog (Rana catesbeiana) pituitary" General and Comparative Endocrinology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto,Toshiharu: "Endothelin B receptor-like immunoreactivity is associated with LHRH immunoreactive fibers in the rat hypothalamus." Neurosience Letters. 223. 117-120 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上村晴子: "アンギオテンシンと後葉ホルモン" Hormone Frontier in Gynecology. 4. 91-102 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto T,Suzuki H,Hirohama T,Nakamura S,Uemura H: "Differential distributions of endothelin A receptor-like and endothelin B receptor-like immunoreactivities in the rat median eminence." Zoological Science. 12 (Suppl). 9 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki H,Yamamoto T,Kikuyama S,Uemura H,et al.: "Distribution of endothelin 3-like immunoreactivity in the gonadotrophs of the female bullfrog" Zoological Science. 13 (Suppl). 12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki H,Yamamoto T,Kikuyama S,Uemura H: "Distribution of endothelin 3-like immunoreactivity in gonadotrophs of the bullfrog (Rana catesbeiana) pituitary." General and Comparative Endocrinology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto T,Suzuki H,Uemura H: "Endothelin B receptor-like immunoreactivity is associated with LHRH immunoreactive fibers in the rat hypothalamus" Neuroscience Letters. 223. 117-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,Hirohumi: "Distribution of endothelin 3-like immunoreactivity in the gonadotroph of the female bullfrog." Zoological Science. 13(Suppl.). 12- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,Hirohumi: "Distribution of endothelin 3-like immunoreactivity in gonadotrophs of the bullfrog (Rana catesbeiana) pituitary." General and Comparative Endocrinology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamamoto,Toshiharu: "Endothelin B receptor-like immunoreactivity is associated with LHRH immunoreactive fibers in the rat hypothalamus." Neuroscience Letters. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Uemura,Haruko: "アンギオテンシンと後葉ホルモン" Hormone Frontier in Gynecology. 4(1). 91-102 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiharu Yamamoto: "Differential distributions of endothelin A receptor-like and endothelin B receptor-like immunoreactivities in the rat median eminence." Zoological Science. 12(Suppl.). 9 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 山 本 利 春: "ラット視床下部におけるエンドセリン受容体A, Bの分布(免疫組織化学による検索)" 日本内分泌学会雑誌. 7. 810 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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