| Project/Area Number |
07640891
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物形態・構造
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| Research Institution | Teikyo Junior College |
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Principal Investigator |
ITA Masatoshi Teikyo Junior College, Depart. of Domestic Science, Lectural, 生活科学科, 講師 (50190674)
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| Co-Investigator(Kenkyū-buntansha) |
ANEYOSHI Mineo Nishitokyo University, Sch. of Sci. and Eng., Prosessor, 理工学部, 教授 (20002339)
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| Project Period (FY) |
1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Oocyte maturation / Starfish / Ovarian follicle cells / 1-Methyladenine / Protein kinase / Hormone and receptor |
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| Research Abstract |
1.Sunstrate for 1-methyladenine biosynthesis
Analyzes using high-performance liquid chromatography of follicel cell extract by reversed-phase and ion-exchange columns indicated a large source of ATP among the adenine-related compounds. GSS caused both 1-MeA production and ATP consumption. The amount of ATP consumed under the influence of GSS was similar to that of 1-MeA produced. These suggest that ATP is a possiblr substrate in the biosynthesis of 1-MeA by starfish ovarian follicle cells.
2.Regulation of 1-methyladenine biosynthesis
Cyclic AMP-dependent protein kinase (PKA) was present in stafish follicle cells. The PKA was activated by cyclic AMP in a dose-dependent manner and 70-kDa protein was phosphorylated. Further studies on the role of the 70kDa protein should provide additional insight into the regular of GSS-stimulated 1-MeA production by starfish follicle cells.
3.Interaction between 1-methyladenine and its receptor
1-Substituted adenines with functional groups bearing electron-releasing or electron withdrawing effect were synthesized and examined effects on starfish oocyte maturation. 1-beta-Nephtylmethyladenine and 1-aminoadenine as agonists of 1-MeA were newly found to induce oocyte maturation. In contrast, 1- (p-nitrobenzyl) adenine and 1-alpha-naphtylmethyladenine as antagonists of 1-MeA inhibited 1-MeA-induced oocyte maturation. Based on these results, we attempt to synthesize 1-MeA derivatives for affinity label to the 1-MeA receptor on oocyte surface.
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