• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A hemoglobin-like protein from Protozoan ciliates : Its structure and function

Research Project

Project/Area Number 07640896
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionTohoku University

Principal Investigator

SHIKWA Kenji  Tohoku Univ.Grad.School of Sci.Professor, 大学院・理学研究科, 教授 (40004337)

Co-Investigator(Kenkyū-buntansha) TAJIMA Gen-ichi  Tohoku Univ.Grad.School of Sci.Research Associate, 大学院・理学研究科, 助手 (00197360)
MATSUOKA Ariki  Tohoku Univ.Grad.School of Sci.Research Associate, 大学院・理学研究科, 助手 (30222293)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsHemoglobin / Myoglobin / Protozoa / Ciliates / Paramecium / Tetrahymena / Autoxidation / Molecular evolution / 絨毛虫類 / シオグロビン
Research Abstract

A myoglobin-like protein from Tetrahymena pyriformis is composed of 121 amino acid residues with a molecular mass of 14,343 daltons including the heme moiety. This is much smaller than sperm whale myoglobin by 32 residues and shows no notable degree of sequence similarity with other myoglobins and hemoglobins. In order to examine this unique protein for its spectral and stability properties, we have isolated a large amount of its native oxy-form directly from the packed cells by using gel filtration on Sephadex G-50 followed by CM-cellulose chromatography. As a result, Tetrahymena oxymyoglobin was found to be quite resistant against the autoxidation reaction over a wide range of pH4-12 in 0.1M buffer at 25゚C,its rate-constant being almost the same as that of sperm whale MbO_2. We therefore conclude that Tetrahymena myoglobin can bind molecular oxygen as stably as mammalian myoglobins can do, in spite of its very contracted structure probably due to a large number of amino acid deletions.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Shikama,K.et al.: "The unique structures of protozoan myoglobin and yeast hemoglobin:An evolutionary diversity." International Journal of Biochemistry and Cell Biology. 27. 1107-1115 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugawara,Y.et al.: "Role of globin moiety in the autoxidation reaction of oxymyoglobin:Effect of 8 M urea." Biophysical Journal. 69. 583-592 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 四釜慶治: "単細胞生物にみられるヘモグロビン様タンパク質-ゾウリムシのMbと酵母のHb-" 生物物理. 35. 13-18 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Korenaga,S.et al.: "A Myoglobin-like Protein from Tetrahymena pyriformis:Isolation and Characterization." Europ.J.Protistol.32. 73-78 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuoka,A.et al.: "The Dimer-Monomer Conversion of Cerithidea Myoglobin Coupled with the Heme Iron Oxidation." Comp.Biochem.Physiol.115B. 483-492 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuruga,M.et al.: "Biphasic nature in the autoxidation reaction of human oxyhemoglobin." Biochim.Biophys.Acta.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 四釜 慶治: "新タンパク質応用工学(分担執筆)" 下等生物にみられるヘモグロビン様タンパク質の構造と機能, 950 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shikama, K.et al.: "The unique structures of protozoan myoglobin and yeast hemoglobin : An evolutionary diversity." International Journal of Biochemistry and Cell Biology. 27. 1107-1115 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sugawara, Y.et al.: "Role of globin moiety in the autoxidation reaction of oxymyoglobin : Effect of 8 M urea." Biophysical Journal. 69. 583-592 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shikama, K.et al.: "TANSAIBOU SEIBUTSU NI MIRARERU HEMOGLOBIN-YOU TANPAKUSHITSU" SEIBUTSU BUTSURI. 35. 13-18 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Korenaga, S.et al.: "A Myoglobin-like Protein from Tetrahymena pyriformis : Isolation and Characterization." Europ.J.Protistol. 32. 73-78 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuoka, A.et al.: "The Dimer-Monomer Conversion of Cerithidea Myoglobin Coupled with the Heme Iron Oxidation." Comp.Biochem.Physiol. 115B. 483-492 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuruga, M.et al.: "Biphasic nature in the autoxidation reaction of human oxyhemoglobin." Biochim.Biophys.Acta. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shikama, K.et al.: KATOU SEIBUTSU NI MIRARERU HEMOGLOBIN-YOU TANPAKUSHITSU NO KOUZOU TO KINOU. SHIN TANPAKUSITSU OUYOU KOUGAKU, 130-139 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shikama, K. et al.: "The unique structures of protozoan myoglobin and yeast hemoglobin : An evolutionary diversity." International Journal of Biochemistry and Cell Biology. 27. 1107-1115 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugawara, Y. et al.: "Role of globin moiety in the autoxidation reaction of oxymyoglobin : Effect of 8 M urea." Biophysical Journal. 69. 583-592 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 四釜慶治 他: "単細胞生物にみられるヘモグロビン様タンパク質-ゾウリムシのMbと酵母のHb-" 生物物理. 35. 13-18 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Korenaga, S. et al.: "A Myoglobin-like Protein from Tetrahymena pyriformis : Isolation and Characterization." Europ. J. Protistol.32. 73-78 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuoka, A. et al.: "The Dimer-Monomer Conversion of Cerithidea Myoglobin Coupled with the Heme Iron Oxidation." Comp. Biochem. Physiol.115B. 483-492 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuruga, M. et al.: "Biphasic nature in the autoxidation reaction of human oxyhemoglobin." Biochim. Biophys. Acta.(in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 四釜 慶治: "新タンパク質応用工学(分担執筆)" 下等生物にみられるヘモグロビン様タンパク質の構造と機能, 950 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 四釜慶治他: "単細胞生物にみられるヘモグロビン様タンパク質" 生物物理. 35(No.3). 13-18 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sugawara, Y. et al.: "Role of Globin Moiety in the Autoxidation Reaction of Oxymyoglobin" Biophysical J.69. 583-592 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shikama, K. et al.: "The Unique Structures of Protozoan Myglobin and Yeast Hemoglobin:: An evolutionary Diversity" Int. J. Biochem. Cell Biol.27. 1107-1115 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 四釜慶治: "下等生物にみられるヘモグロビン様タンパク質の構造と機能" 新タンパク質応用工学(分担執筆). 130-139 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi