Molecular evolution of MADS genes, and origin and evolution of flowers

Research Project

Project/Area Number	07640924
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	系統・分類
Research Institution	CHIBA UNIVERSITY
Principal Investigator	ITO Motomi Chiba Univ., Faculty of Science, Associate Professor, 理学部, 助教授 (00193524)
Project Period (FY)	1995 – 1996
Project Status	Completed (Fiscal Year 1996)
Budget Amount *help	¥2,000,000 (Direct Cost: ¥2,000,000) Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000) Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords	Angiosperms / Ginkgo / Houttuyniaceae / Magnoliaceae / MADS gene / Ranunculaceae / MADS-box遺伝子 / 花 / 形態形成遺伝子 / MADS-box / floral organ gene / Angiosperms
Research Abstract	Angiosperms is characteristic by having a flower which consists of sepals, petals, stamens and gynoecium. The merphology of flower is diversified in several lineages of angiosperms. The purpose of present study is clarified the origin of flower and its evolution. For this purpose, I have performed a cloning and analysis of MADS-box gene family which is very important in organogenesis of flower. From angiosperms, Magnolia, Houttuynia, Ranunculus, Anemone, and Clematis were selected as materials, and Ginkgo from gymnosperms. As a result using RT-PCR method, 15,12,14,10,13,5 clones of MADS-box genes are cloned from these plants respectively. These clones are determined their DNA sequences and I constructed phylogenetic tree of these genes together with the other known MADS-box genes. The tree indicated that the clones from the same plants do not make a monophyletic clade, but the sequences from the different plants clustered together. And in some gene group, the occurrence of gene duplication is suggested in the way of evolution from gymnosperms to angiosperms.