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Development and Application of a Novel Bioreactor with Loofa (Luffa cylindrica) Sponge as a Carrier For Cell Immobilization.

Research Project

Project/Area Number 07650946
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物・生体工学
Research InstitutionUniversity of Tsukuba

Principal Investigator

OGBONNA James chukwuma  University of Tsukuba, Institute of Applied Biocemistry Associate Professor, 応用生物化学系, 講師 (00261777)

Co-Investigator(Kenkyū-buntansha) TANAKA Hideo  University of Tsukuba, Institute of Applied Biocemistry Professor, 応用生物化学系, 教授 (40015657)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsLoofa spong / Cell Immobilization / Immobilization carrier / Flocculating cells / Non-flocculating cells / Filamentous cells / Aerobic fermentation process / Anaerobic fermentation process / 充填層 / アミラーゼ生産 / 細胞固定化 / 物質生産
Research Abstract

Efficient immobilization of flocculating cells can be achieved by simply inoculating a reactor containing a bed of the loofa sponge with the cell suspension. The cells were trapped inside the lattice of the sponge and grew to high cell densities. In the case of non-flocculating cells, flocculation was induced by addition of flocculating agent (chitosan) and the flocculated cells were efficiently immobilized as described above. Fungi was immobilized by inoculating either spores or homogenized hyphae into a reactor containing a bed of the loofa sponge. The spores germinated inside the lattice structure of the sponge and the resulting hyphae were entangled (immobilized) on the sponge.
Although mass transfer was better in a fixed bed made of a single cylindrical sponge, the amounts of sponge per unit reactor volume and the immobilized cells per unit sponge were higher when sliced loofa sponges were used for bed construction. Thus cylindrical or sliced loofa sponges should be used for aerobi … More c or anaerobic fermentation processes, respectively.
As examplex of application to anaerobic fermentation, immobilized flocculating (Saccharomyces cerevisiae) or non-flocculating (Candida brassicae) was used for continuous ethanol production from molasses and sucrose medium. The processes were stable for long periods of time. In the case of aerobic processes, long term continuous glucoamylase production by Aspergillus awamori immobilized on loofa sponge was possible. Furthermore, continuous direct ethanol production from starch was achieved by using a combination of immobilized Aspergillus awamori and Saccharomyces cerevisiae.
The above results have shown that representative flocculating and non-flocculating fermentation microorganisms (yeast, bacteria and fungi) can be efficiently immobilized on loofa sponge. Furthermore, by using either the cylindrical or sliced loofa sponge, suitable fixed beds for aerobic or anaerobic fermentation processes can be constructed. It can be thus concluded that bioreactor with loofa sponges has great potential for application in a wide range of fermentation processes. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ogbonna,James C.: "Loofa (Luffa cylindrica) sponge as a carrier for microbial cell immobilization." J.Fermentation and Bioengineering. 78. 437-442 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogbonna,James C: "Efficient production of fuel ethanol by cells immobilized on loofa (Luffa cylindrica) sponge." Biotechnology Letters. 印刷中 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogbonnna, James C.: "Development of a method for immobilization of nonflocculating cells in loofa (Luffa cylindrica) sponge." Process Biochemistry. 31. 737-744 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 冨山晶大: "細胞をヘチマ繊維体に固定化した培養系による効率的なエタノール生産" 日本生物工学会九州大会講演要旨集. 172- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 田中豊: "糸状菌のヘチマ繊維体への固定化方法と好気的プロセスへの応用" 日本生物工学会名古屋大会講演要旨集. 75 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogbonna, J.C., Liu, Y.C., Liu, Y.K., and Tanaka, H.: "Loofa (Luffa cylindrica) sponge as a carrier for microbial cell immobilization." J.Ferment.Bioeng.78. 437-442 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomiyama, S., Ogbonna, J.C., and Tanaka, H.: "Efficient ethanol production with cells immobilized in loofa sponge" Annual meeting of the society for fermentation and Bioengineering, Japan. (Kyushu). Abstract No 524. 172 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogbonna, J.C., Tomiyama, S., and Tanaka, H.: "Development of a method for immobilization of nonflocculating cells in loofa (Luffa cylindrica) sponge." Proc.Biochem.31. 737-744 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanaka Y., Ogbonna, J.C., and Tanaka, H.: "Immobilization of fungi in loofa sponge and its application to aerobic fermentation." Annual meeting of the society for fermentation and Bioengineering, Japan. (Nagoya). Abstract No 329. 75 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogbonna, J.C., Tomiyama, S., Liu Y.C., and Tanaka, H.: "Efficient production of fuel ethanol by cells immobilized on loofa (Luffa cylindrica) sponge." Biotechnol. Lett.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ogbonna,James C: "Development of a method for immobilization of nonflocculating cells in loofa(Luffa Cylindrica)sponge." Process Biochemistry. 31. 737-744 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中豊: "糸状菌のヘチマ繊維体への固定化方法と好気的プロセスへの応用" 日本生物工学会名古屋大会講演要旨集. 75 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ogbonna,James C: "Efficient production of fuel ehanol by cells immobilized on loofa(Luffa Cylindrica)sponge." Biotechnology Letters. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] James C. Ogbonna: "Development of a method for Immobilization of Non-flocculating Cells in Loofa(Luffa cylindrica)Sponge." Process Biochemistry. 31. (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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