| Project/Area Number |
07650967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | UNIVERSITY OF EAST ASIA |
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Principal Investigator |
HORIUCHI Takashi UNIVERSITY OF EAST ASIA ENGINEERING PROFESSOR, 工学部, 教授 (10201758)
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| Co-Investigator(Kenkyū-buntansha) |
MURABAYASHI Shun Hokkaido Univ., Graduate School, Assoc. Prof., 大学院, 助教授 (30200306)
OZAKI Masayori Univ. of East Asia, Graduate School, Prof., 大学院, 教授 (00039588)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | peritoneal dialysis / peritoneal fibrosis / peritoneal mesothelial cell / DNA synthesis / glucose / albumin / membrane permeability / chemical fibrosis |
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| Research Abstract |
Incidence of sclerosing encapsulating peritonitis (SEP) increases as a duration of peritoneal dialysis is prolonged. It has been hypothesized that hydrated degeneration of collagen matrix is possibly occurred at high concentration of glucose and it may result in reducing cell growth in the mesothelium.
To understand both mechanism of regeneration of the mesothelial cell under high permeable state and change in peritoneal permeability under chemically induced peritonitis, in vitro experiments with cultured mesothelial cells and in vivo experiments with intraperitoneal injection of Chlorohexidine gluconate (CHG) were carried out. After one to five week exposure of type I collagen coated multi-titer plate. to varying concentrations of glucose, growth of mesothelial cells seeded were suppressed at 2g/dl of albumin supplement no matter what concentration of glucose supplemented was. Without high concentration of albumin, DNA synthesis was in a dose-dependent manner of glucose. There was no significant difference of DNA synthesis between groups with and without one-week glucose immerse when mesothelial cells were cultured only 10%FCS/MEM.
20ml/kg of 0.08-0.2wt% CHG was injected into the abdominal space of male Wistar rat (at 8 weeks). Eight out of 11 rats were survived and 5 out of 8 rats developed adhesions and one developed SEP-like degeneration. Although there was no statistically signifficant change, MTAC increased from 1.18(]SY.+-。[)0.66ml/min to 1.92(]SY.+-。[)0.6ml/min in the first three weeks (p=0.09) and slightly decreased at the 4th week.
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