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Studies on Evolution of Mutator transposable element in Grass Family

Research Project

Project/Area Number 07660002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHirosaki University

Principal Investigator

ISHIKAWA Ryuji  Hirosaki Univ.Faclty of Agri., Associate Prof., 農学部, 助教授 (90202978)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsOryza sativa / Transposon / Mutator / RFLP / transposase / Isozyme / mutable strain / 係 / アイソザイム / Oryza sativa / Mu / 同祖性因子
Research Abstract

Rice homolog to maize Mutator transposable element was cloned, which has similarity to transposase (TNP) domain of the Mutator element in maize. The exact domain consisting of 426bp is capable to translate into142 amino acids. and share 69% homology at DNA level and 80% at amino acids level. Rice cultlvars carry at least three copies in their genomes and wild relatives carry different number of copies and RFLP patterns. Expression of the domain was confirmed by RT-PCR with Japonica strains but not in lndica strains at seedling and mature leaf stage. Mutable strains were generated through self pollination from a famous Hokkaido's local cultivar, which showed some mutants such as albino, chimera and twin embryo. They also presented RFLPs probed with rice TNP-like domain. There is no reason to believe pollen and other contamination sources for the RFLPs because the progeny was self-pollinated through several generations and stil carried recessive dwarf-1 gene as homozygous condition. And we also obtalned albino, chlorina and twin embryo mutants from the progeny. Other progenies from Akage are also known to carry several mutant genes generated spontaneously through self-pollination such as awnless-1, awnless-2, dwarf-2 and Undulated rachis-1 for one century. Such mutabllity affected to plant breeding, which enlarge their cultivated area up to more north region. Other sources as mutable populations will be discussed. The populations generated from subspecies level-crosses like a hybrid dysgenesis system in Drosophila. At least 0.5% mutants were generated in their progenies. The ratio would be 1/20 genes related to those chlorosis phenomena per generation.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ishikawa, R.: "The report of 5th International Symposium on Rice Molecula Biol." RGN. 13(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, R.: "Putative transposase like doma in found in rice genoml" RGN. 13(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, R.: "Mutator like transposase domain found in rice genoml" Proceed. of 5th Intnl. Symp. on Rice Mol. Biol.(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, R: "The report of 5th Internatoinal Symposium or Rice Mdecular Biology" RGN. B(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, R.: "Putative trans posase like donain found in rice genome" RGN. B(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, R.: "Mutator like transposase domain found in rice genome" Proceed.of 5th Intnl.Symp.on Rice Mol.Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa: "The report of Sth Interationul Symposium on Rice Moleculm Biobogy" RGN. 13. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa: "Rutative transposcse like domain foumd in rice genome" RGN. 13. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa: "Mututor like transposase domuin fornd in rice genome" Proceed. of the Sth Intnl. Symp. on Rlce Md. Biol.(1997)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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