Molecular based analyzes of distantly related interspecific and intergeneric hybrids (including somatic hybrids) for useful gene cloning

• Project/Area Number: 07660004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: YAMAGATA UNIVERSITY
• Principal Investigator: IMANISHI Shigeru Yamagata University, Faculty of Agriculture, Professor, 農学部, 教授 (40007084)
• Co-Investigator(Kenkyū-buntansha): EGASHIRA Hiroaki Yamagata University, Faculty of Agriculture, Research assistant, 農学部, 助手 (60223632)
• Project Period (FY): 1995 – 1996
• Project Status: Completed (Fiscal Year 1996)
• Budget Amount: ¥1,900,000 (Direct Cost: ¥1,900,000); Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000); Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: Lycopersicon esculentum / Lycopersicon peruvianum / Lycopersicon chilense / Solanum lycopersicoides / interspecific hybrid / somatic hybrid / molecular marker / adventitious shoot formation ability / 遠縁交雑 / 分子マーカー / インベルターゼ遺伝子 / GISH / 酸性ホスファターゼ遺伝子 / トマト栽培種 / トマト野生種L.peruvianum / RAPDマーカー / 苗条再分化能 / 灰色カビ病 / 立性形質

Research Abstract

Genetic polymorphisms between tomato cultivar Lycopersicon esculentum and the remote wild species should be employed for the molecular genetic analysis of useful traits. The present study reports : (1) Efficiency of ovule selection culture method for the hybridization between tomato and 'peruvianum-complex' along with the development of plant materials for this study : (2) Identification of acid invertase gene for sucrose accumulation in backcross generations B_2, B_3 and B_4 from the cross of tomato and L.peruvianum var.humifusum by using PCR gene diagnosis : (3) Skewed segregation of molecular markers (RAPD,invertase gene and acid phosphatase gene) in backcross generations B_1, and B_2 : (4) Two closely linked RAPD markers and invertase gene located on chromosome 3 to high adventicious shoot formation gene which harbors in L.chilense PI128644, were discovered from the analysis of B_1 and B_2 : (5) Analysis of chloroplast DNA and nuclear genome of somatic hybrid and progenies between tomato and Solanum lycopersicoides by RFLP and GISH.

Research Products

• [Publications] IMANISHI SHIGERU: "Development of interspecific hybrids between Lycopersicon esculentum and L.peruvianum var.humifusum and introgression of L.peruvianum invertase gene" Breeding Science. 46. 356-359 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] TAKASHINA TADASHI: "Evaluation of the cross-incompatibility of 'peruvianum-complex' lines with Lycopersicon esculentum Mill.by the ovule selection method" Breeding Science. 47. 33-37 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] IMANISHI SHIGERU: "Development of interspecific hybrids between Lycopersicon esculetum and L.peruvianum var.humifusum and introgression of L.peruvianum invertase gene into L.esculentum" Breeding Science. 46. 356-359 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] TAKASHINA TADASHI: "Evaluation of the cross-incompatibility of 'peruvianum-complex' lines with Lycopersicon esculentum Mill.by the ovule selection method" Breeding Science. 47. 33-37 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] TAKASHINA TADASHI: "Segregation of high adventitious shoot formation ability and wild species-specific RAPD marker in BC_2F_1 progeny from interspecific hybrid between tomato and a wild species L.chilense" Breeding Science. 47(Supple.1). (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] IMANISHI SHIGERU: "Introgression of L.peruvianum invertase gene into L.esculentum" Breeding Science. 46. 356-359 (1996)
• [Publications] TAKASHINA TADASHI: "Evaluation of the cross-incompatibility of 'peruvianum-complex' lines with tomato by the ovule selection method" Breeding Science. 47. 33-37 (1997)