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Mapping and functional analysis of nucleus-cytoplasm compatibility gene in wheat

Research Project

Project/Area Number 07660007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKobe University

Principal Investigator

NAKAMURA Chiharu  Kobe University, Dept.of Professor, 農学部, 教授 (10144601)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsNucleus-cytoplasm compatibility gene (Ncc) / Triticum timopheevi / Aegilops squarrosa / Tetraploid wheat / Mapping / RAPD markers / Trifieum timopheevi / 細胞質ゲノム / 核ゲノム / 核細胞質親和性 / Aegilops squrrosa
Research Abstract

An alien nucleus-cytoplasm compatibility gene from Triticum timopheevi (Ncc-tmp) was introduced into tetraploid wheat, Triticum durum, with the cytoplasm of Aegilops squarrosa. The following results were obtained using this nucleus-cytoplasm (NC) hybrid.
1) Ncc-tmp controls gametophytic male fertility and zygotic viability of the NC hybrids under hemizygous condition.
2) 4 RAPD markers that are closely linked with each other and with Ncc-tmp were selected, and using a series of D genome chromosome substitution lines Ncc-tmp was mapped on chromosome 1A.
3) Sequences adjacent to Ncc-tmp was conserved uniquely in timopheevi group of wheat, and this sequences were also present in Triticum urartu, a A genome donor for tetraploid and hexaploid wheat.
4) Segregation analysis of plump and shriveled seeds indicated the presence of two independent functional Ncc-tmp in T.timopheevi.
5) NC hybrid-specific polymorphisms were detected in mitochondrial atp6-rps13 and nad3-orf156 by SSCP analysis and PCR-restriction enzyme analysis. These results set basis for fine mapping and cloning of Ncc genes that are the key genes controlling compatibility between nuclear genome and cytoplasmic genomes in the tribe Triticeae.
6) In addition, NC hybrids of common wheat cv Chinese Spring with cytoplasms of 46 species from Triticum and Aegilops were studied to examine nuclear and cytoplasmic polymorphisms that are related to NC interactions. The study showed that in certain NC hybrids nuclear polymorphisms exist and are fully transmitted through all gametes. In one NC hybrid line, it was suggested that the cytoplasmic genome type was changed into the paternal genome type. These polymorphisms might be caused by NC interaction, thus leading to NC compatibility.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Asakura, N.: "RAPD markers linked to the nuclear gene from Triticum timopheevii that confers compatibility with Aegilops squarrosa cytoplasm on alloplasmic durum wheat" Genome. 40 (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Asakura, N.: "A nuclear compatibility gene, Ncc-tmp, of Triticum timopheevi for the cytoplasm of Aegilops squarrosa" Genes & Genetic Systems. 72 (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 朝倉史明: "チモフィーピ系コムギの保有するタルホコムギ細胞質親和性核遺伝子のマッピング" 近畿作物・育種研究. 42 (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Asakura, N., Nakamura, C.and Ohtsuka, I.: "RAPD markers linked to the nuclear gene from Triticum timopheevi that confers compatibility with Aegilops squarrosa cytoplasm on alloplasmic durum wheat" Genome. 40 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Asakura, N., Nakamura, C.and Ohtsuka, I.: "A nuclear compatibility gene, Ncc-tmp, of Triticum timopheevi for the cytoplasm of Aegilops squarrosa" Genes & Genetic Systems. 72 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Asakura, N., Nakamura, C., Ohtsuka, I.and Mori, N.: "Mapping of Ncc-tmp of Triticum timopheevi for the cytoplasm of Aegilops squarrosa (in Japanese)" Kinki Sakumotsu-Ikushu Kenkyu. 42 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Asakura,N.: "RAPD markers linked to the nuclear gene from Triticum timopheevii that confers compatibility with Aegilops squarrosa cytoplasm on alloplasmic durum wheat" Genome. 40(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Asakura,N.: "A nuclear compatibility gene,Ncc-tmp,of Triticum timopeevi for the cytoplasm of Aegilops squarrosa" Genes & Genetic Systems. 72(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 朝倉史明: "チモフィービ系コムギの保有するタルホコムギ細胞質親和性核遺伝子のマッピング" 近畿作物・育種研究. 42(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Asakura, N. et al.: "Random amplified polymorphic DNA markers linked to the nuclear gene (s) from Triticum timopheevi that confer compatibility with Aegilops squarrosa cytoplasm on durum wheat" Plant Genome IV, San Diego. 58- (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 朝倉史明他: "Aegilops squarrosa細胞質に対するTriticum timopheevi由来親和性核遺伝子のマッピング 4.連鎖RAPDマーカーの同定" Breeding Science. 45. 188- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 朝倉史明他: "Aegilops squarrosa細胞質に対するTriticum timopheevi由来親和性核遺伝子のマッピング 3.連鎖RAPDマーカーの4倍性、6倍性コムギにおける分布" Japanese Journal of Genetics. 70. 720- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 勝田雅大他: "Triticum, Aegilops属の細胞質を持つパンコムギ核細胞質雑種の核ゲノム多型" Japanese Journal of Genetics. 70. 720- (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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