| Project/Area Number |
07660047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Ishikawa Agricultural college |
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Principal Investigator |
KANO Yasutaka Ishikawa Agric.Coll., Bio.Pro., Professor, 生物生産学科, 教授 (80115823)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Kagafutokyuri / Bitterness / Cucurbitacin C / Age of leaf and fruit / Totalnitrogen / Amino acid-nitrogen / アミノ酸態窒素含量 / 酵素遺伝子 / 酵素 遺伝子 / 苦味 / 全窒素含量 / 硝酸イオン含量 / アミノ酸態窒素 / 栄養生長 / 苦味系統 / 無苦味系統 / 整枝 / 台木 / 草勢 / 硝酸態窒素 / 総窒素 |
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| Research Abstract |
The development of bitterness in the leaf and fruit of cv. Kagafutokyuri was investigated.
Results in 1995
(1). Bitter fruits were borne on the first lateral shoot, but not on the secondary lateral one.
(2). The bitterness was strong in young leaf and fruit, but it decreased with age.
(3). The total leaf nitrogen content of the bitter line was equal to that of the non-bitter line but the leaf nitrate ion content of the former was less than half of the latter.
Results in 1996
(1). A larger number of bitter fruit was observed in the bitter line than in the non-bitter line
(2). The content of total nitrogen and amino acid-nitrogen in the leaf were higher in the bitter line than in the non-bitter line.
(3). The content of total nitrogen and amino acid-nitrogen was higher in the upper leaf (or the bitter fruit) than in the lower(or non-bitter) one.
Results in 1997
(1). The occurrence of bitter fruit was high in the early growth stage, as compared with the late growth stage. The content of total nitrogen, nitrate ions and amino acid nitrogen was higher in the early growth stage than in the late growth stage.
(2). The occurrence of bitter fruit was high in the early planting, as compared with the late planting. The content of total nitrogen, nitrate ions and amino acid nitrogen was higher in the early planting than in the late planting.
From these results, it can be considered that the higher content of amino acid-nitrogen in the leaf of the bitter line, of bitter leaf, or of the bitter fruit results in a large quantity of protein production, which causes active synthesis of enzymes related to the synthetic pathway of cucurbitacin C,causing intense bitterness.
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