| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Research Abstract |
1.A highly-sensitive detection system for peroxides in a lipid fraction from an insect was developed. The system employs a high performance liquid chromatography and a chemiluminescence detector (CL-HPLC). The detection limit for triglyceride peroxide was 1 pmol. Samples from an insect body were found to contain unknown inhibitor(s) for chemiluminescence, but it was efficiently removed by a pretreatment with Sep-pak NH_2.
2.The larvae of the onion fly, Delia antiqua was fed with a diet containing a phototoxicant, Erythrosine B.When the larvae was given an oxidative stress by illuminating them with a fluorescent lamp, the larvae contained a larger amount of lipid peroxide compared with the control insects (without Erythrosine B).
3.The role of uric acid against the oxidative stress was examined using the silkworm, Bombyx mori. When an inhibitor of xanthine dehydrogenase, allopurinol, was added to the artificial diet of B.mori, the amount of uric acid in the body of B.mori grown on this diet was decreased to a nondetectable level. Under illumination with ultraviolet light, the silkworms depleted with uric acid showed a significantly shorter life span than the normal insects. The silkworms depleted with uric acid also showed decreased resistance against oxidative stress produced by a phototoxicant, rose bengal.
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