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Studies on the physiological mechanisms of isoflavonoids on the nod-gene expression of Bradyrhizobium japonicum

Research Project

Project/Area Number 07660086
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YAMAKAWA Takeo  Kyushu University, Faculty of Agriculture, Associate Professor., 農学部, 助教授 (20220238)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRj-gene / Bradyrhizobium japonicum / nod-gene / isoflavonoids / glyceollins / genistein / daidzein / Nod metabolites / ジェニスタイン / 根粒形成遺伝子 / ファイトアレキシン / グリセオリン / グリシノール
Research Abstract

In order to investigate the point shared into ineffective or effective root nodules when soybean cultivars harboring Rj-gene were inoculated with a compatible or incompatible strain, we analyzed anatomically the nodule development in the roots of Rj_2Rj_3-and Rj_4-genotype soybean, CNS and Hill respectively, infected by Bradyrhizobium japonicum strains Is-1 (incompatible to CNS and compatible to Hill) and Is-34 (compatible to CNS and incompatible to Hill). The root hairs curlings were assayd by the staining method with methylene blue, the cortex divisions were examined with a light microscope, the invitation of rhizobia were investigated by the fluorescent antibody of rhizobia strains Is-1 or Is-34 with a fluorescence microscope. These results showed that the restriction mechanism of the infection with incompatible strains might operate at the step of rhizobia invasion into the primary meristem directly under cortex within 5 days after sowing and inoculation.
On the assumption that the … More various composition of isoflavonoids were different in those soybean roots and the species of nod metabolites produced by genistein, daidzein and the root extracts were different among rhizobia strains, we searched a signal molecular which operate at the point shared intoineffective or effective root nodules. Daizein and glyceollin I contents in those soybean roots were level up to 7 days. Also the roots of CNS and Hill of 3 and 5 days' seedling inoculated with Is-1 or Is-34 were no difference in the contects of genistein and daidzen known as main isoflavonoids which induced the nod-gene expression of rhizobia. However a specific fluorescent spot was identified in the root extract of Hill and not in that of CNS.
In order to analyze the composition and amount of nod metabolites, Is-1 and Is-34 were incubated using ^<14>C-acetate as tracer with genistein, daidzein, each soybean root extract or a specific fluorescent spot of Hill. These results shown that the separation pattern of nod metabolites of Is-1 or Is-34 were little difference among each treatment, but in comparison between Is-1 or Is-34, the number and intensity of nod metabolites were large difference. The chitinase treatment of those nod metabolites decreased the number of nod metabolites, thus it was confirmed that nod factors were included those nod metabolites. Hence, it was the different composition of nod metabolites between strains determined the compatibility of soybean varieties and B.japonicum strains, through different responses of host plant roots to nod metabolites. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Yuichi Saeki,Takeo Yamakawa,Motoki Ikeda,Junji Ishizuka: "Effects of Roots exudatgs of Rj_2Rj_3-and Rj_4-genotype Soybeans on growth and Chemotaxis of Brady rhizobium japonicum." Soil Science and Plant Nutrition. 42・2. 413-417 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimasa Nakano,Takeo Yamakawa,Motoki Ikeda,Junji Ishizuka: "Nodulation of Rj-Soybean Varieties with Rhizoblum fredii VSDA 193 under limited Supply of Nutrients." Soil Science and Plant Nutrition. 43(In press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Saeki, Y., Yamakawa, T., Ikeda, M.and Ishizuka, J.: "Effects of roots exudates of Rj_2Rj_3-and Rj_4-genotype soybeans on growth and chemotaxis of Bradyrhizobiium japonicum." Soil Sci.Plant Nutr.42-2. 413-417 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakano, Y., Yamakawa, T., Ikeda, M.and Ishizuka, J.: "Nodulation of Rj-soybean varieties with Rhizobium fredii USDA 193 under limited supply nutritients." Soil Sci.Plant Nutr.43(In Press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yuichi Saeki,Takeo Yamakawa,Motoki Ikeda,Junji Ishizuka: "Effects of roots exudates of Rj_2 Rj_3-and Rj_4-genotype soybeans on growth and chemotaxis of Bradyrhizobium japonicum." Soil Science and Plant Nutrition. 42・2. 413-417 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yuichi Saeki, Takeo Yamakawa, Motoki Ikeda, Junji Ishizuka: "Effects of root exudates of Rj_2 Rj_3-and Rj_4-genotype Soybeans on growth and Chemotaxis of Brady rhigobium Japonicum." Soil Science and Plant Nutrition. 42(In Press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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