| Project/Area Number |
07660107
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | MIE UNIVERSITY |
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Principal Investigator |
TAGUCHI Hiroshi MIE UNIVERSITY,BIORESOURCES,PROFESSOR, 生物資源学部, 教授 (60024593)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | NAD / DNA / lymphocyte / nicotinic acid / nicotinamide / MNNG / DNA repair / poly (ADP-ribose) polymerase / ナイアシン / FACS / フローサイトメーター / DNA鎖の損傷 / ヒトリンパ球 / PARP |
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| Research Abstract |
The effect of niacin on NAD content and on DNA repair in human lymphocytes was investigated. Lymphocytes were incubated in culture medium supplemented with various concentrations of nicotinic acid or nicotinamide. Incubation of lymphocytes with nicotinic acid at 5 muM or nicotinamide at 10 mM resulted 2-3 fold increase in NAD content. Under these conditions lymphocytes were treated with N-methyl-N-nitro-N-nitrosoguanidine (MNNG) to investigate the effect of niacin on DNA repair synthesis. Interestingly, the rejoining of DNA strand breaks was promoted by nicotinic acid but nicotinamide inhibited the rejoining. The dramatic degradation of NAD was observed when lymphocytes were treated with MNNG after elevating NAD level in the cells with nicotinic acid but not with nicotinamide. These results suggest the possible role of poly (ADP-ribose) polymerase to DNA repair.
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