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Regulation of artery, immune and nervous systems by albutensin derived from serum albumin

Research Project

Project/Area Number 07660162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YOSHIKAWA Masaaki  Faculty of Agriculture, Kyoto University, Associate Professor, 農学部, 助教授 (50026572)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsserum albumin / albutensin / complement / tumour necrosis factor / phagocytosis / bombesin / food intake / neuropeptide Y / 補体C3a / 補体C5a / 免疫促進
Research Abstract

1. Bovine albutensin A (Ala-Leu-Lys-Ala-Trp-Ser-Val-Ala-Arg) was isolated from the tryptic digest of bovine serum albumin as an ileum-contracting peptide. Human and porcine counterparts (Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg and Ala-Phe-Lys-Ala-Trp-Ser-Leu-Ala-Arg) were also ileum-contracting peptides. These peptides were agonists for complement C3a and C5a. They stimulated phagocytosis and induction of TNF.
2. Bovine albutensin C (Arg-His-Pro-Glu-Tyr-Ala-Val-Ser-Val-Leu-Leu-Arg) was also isolated from the tryptic digest of bovine serum albumin as an ileum-contracting peptide. Human and porcine counterparts did not show the same activity. Albutensin C was also an agonist for C3a receptor and stimulated phagocytosis.
3. Human albutensin A showed affinity for bombesin receptor (IC_<50>=133muM). This peptide stimulated food intake at a dose of 10nmol (icv.) in fasted mice. However, it suppressed food intake at a dose of 30 nmol. Human albutensin A seems to be a partial agonist for bombesin receptor because bombesin suppress food intake. On the other hand, synthetic human des-Arg-albutensin A-NH_2 also showed affinity for bombesin receptor and supressed food intake at 100 nmol. It didn't stimulate food intake and seems to be a bombesin agonist. Bovine and porcine albutensin A did had no effect on food intake because their affinities for bombesin receotor were low.
4. A synthetic fragment peptide of bovine serum albumin, Tyr-Leu-Ser-Leu-Ile-Leu-Asn-Arg showed affinity for neuropeptide Y_2 receptor. This peptide was named albutensin B.Albutensin might be an antagonist for neuropeptide Y because it suppressed food intake in fasted mice after icv.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] M.Takahashi: "Studies on the ileum-contracting mechanism and identification as a complement C3a receptor agonist peptide of oryzatensin." Peptides. 17(1). 5-12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Fujita: "Vasorelaxation by casomokinin L, a derivative of β-casomorphin and casoxin D, is mediated by NK_1 receptor." Peptides. 17(4). 635-639 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Takahashi: "Idetification of casoxin C, an ileum-contracting peptide derived from bovine κ-casein, as an agonist for C3a receptors." Peptides. 18(3). in press (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Takahashi: "Albutensin A, an ileum-contracting peptide derived from serum albumin, acts through both receptors for complement C3a and C5a." Lett. Peptide Sci.in press (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 吉川正明: "学習能を高めるペプチド" バイオサイエンスとインダストリー. 54(3). 205-205 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 吉川正明: "食品タンパク質からの生理活性ペプチドの生産とプロテアーゼの選択" 酪農科学・食品の研究. 45(3). A51-A60 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Takahashi et al.: "Studies on the ileum-contracting mechanism and identification as a complement C3a receptor agonist peptide of oryzatensin." Peptides.17 (1). 5-12 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Fujita et al.: "vasorelaxation by casomokinin L,a derivative of beta-casomorphin and casoxin D,is mediated by NK_1 receptor." Peptides.17 (4). 635-639 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Takahashi et al.: "Identification of casoxin C,an ileum-contracting peptide derived from bovine kappa-casein, as an agonist for C3a receptors." Peptides.18 (3)(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Takahashi et al.: "Albutensin A,an ileum-contracting peptide derived from serum albumin, acts through both receptors for complement C3a and C5a." Lett.Peptide Sci.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Yoshikawa: "A peptide improving learning." Bioscience annd Industry. 54 (3). 205-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Yoshikawa: "Selection of proteases for the production of bioactive peptides derivved from food" Jap.J.Dairy Food Sci.45 (3). A51-A60 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Takahashi: "Studies on the ileum-contracting mechanism and identification as a complement C3a receptor agonist peptide of oryzatensin." Peptides. 17(1). 5-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Fujita: "Vasorelaxation by casomokinin L,a derivative of β-casomorphin and casoxin D,is mediated by NK_1 receptor." Peptides. 17(4). 635-639 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川正明: "学習能を高めるペプチド" バイオサイエンスとインダストリー. 54(3). 205-205 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川正明: "食品タンパク質からの生理活性ペプチドの生産とプロテアーゼの選択" 酪農科学・食品の研究. 45(3). A51-A60 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川正明: "食品中のタンパク質・ペプチド・アミノ酸の生体調節機能" BIO Clinica. 11(11). 33-37 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川正明: "食品蛋白質から多様な生理活性ペプチドが派生する" 医学のあゆみ. 180(6). 378-379 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉川正明: "生物化学実験法38,食品中の生体機能調節物質研究法" 学会出版センター, 200 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Yoshikawa: "Peptide Chemistry 1996" Protein Research Foundation, 543 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Yoshikawa: "Peptide Chemistry 1995" N. Nishi, 556 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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