| Project/Area Number |
07660379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MANABE Noboru Kyoto University, Department of Animal Science, Associate Professor, 農学部, 助教授 (80243070)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | apoptosis / granulosa cells / cumulus cells / oocytes / ovarian follicular atresia / proteases / monoclonal antibodies / cell membrane glycoprotein-receptors / 卵胞選択的死滅 / プログラム細胞死・アポトーシス / ブタ卵巣 / TUNEL組織化学 / レシチン細胞化学 / 細胞膜結合糖鎖 |
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| Research Abstract |
The porcine antral follicles were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation estimated by electrophoresis were noted only in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.
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