| Project/Area Number |
07660405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Miyazaki University |
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Principal Investigator |
HORII Yoichiro MIYAZAKI UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (80173623)
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| Co-Investigator(Kenkyū-buntansha) |
MAKIMURA Susumu MIYAZAKI UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (80003127)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Strongyloides / mast cell / gastrointestinal tract / mucosa / protective mechanism / goblet cell / chymase / typtase |
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| Research Abstract |
Adult worms of canine Strongyloides (Strongyloides stercoralis), bovine Strongyloides (S.papillosus) and porcine Strongyloides (S.ransomi) were intraduodenally inoculated into W/W^V and +/+ mice. The expulsion of S.stercoralis from W/W^V mice was significantly slower than that from +/+ mice. While S.papillosus and S.ransomi were expelled immediately and there were no differences between W/W^V mice and +/+ mice. When S.papillosus adult worms were intraduodenally transferred into other rodent than mice, the worms highly established in hamsters and Mongolian gerbils but not in rats. However, after establishment of S.papillosus adult worms, it caused paralytic ileus and host death in the gerbils. Detailed mechanisms of this paralytic ileus are now under investigation.
We succeeded cDNA clonings of a tryptase and 2 chymases form mast cells of the intestinal mucosae of Mongolian gerbils. When the sequences and the expression in the tissues of these proteases were compared to those of rats and mice, the proteases from gerbil mast cells were similar to those of connective tissue mast cells of rats and mice. RNA blotting revealed that the mast cell proteases of gerbils were not identical between skin mast cells and mucosal mast cells.
There is a possibility that the expulsive mechanism (s) of S.papillosus and S.ransomi is different from that of S.ratti, S.venezuelensis and S.stercoralis.
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